【作者】 王一博； 孙睿洁； 冀林璞； 刘乔瑞； 郑茜月； 崔玲玲；

【Author】 Wang Yibo;Sun Ruijie;Ji Linpu;Liu Qiaorui;Zheng Xiyue;Cui Lingling;School of Public Health, Zhengzhou University;

【机构】 郑州大学公共卫生学院；

【摘要】 目的评价亚麻酸复合粉(亚麻籽微囊粉、秋葵粉、桑叶粉)对高脂饮食诱导所致雄性大鼠生殖损伤及血脂改善方法 90只8周龄雄性SD大鼠随机分为6组,每组15只,普通饲料喂养空白对照组(CON组),高脂饲料喂养模型组(HFD组)、亚麻酸复合粉低中高各剂量组(LLA/MLA/HLA组)和辛伐他汀阳性对照组(PC组),高脂饲料造模4周后连续灌胃复合粉4周。观察记录各组大鼠体重、附睾及睾丸重量,检测精子浓度及活力,检测血脂指标及抗氧化水平。结果与HFD组相比,亚麻酸复合粉各剂量组大鼠睾丸和附睾重量。与HFD组相比,亚麻酸复合粉各剂量组和PC组精子活力和精子活率升高,精子死亡率降低,差异均有统计学意义(P均<0.05);亚麻酸复合粉干预可以提高抗氧化能力及改善大鼠血脂水平。结论高脂饮食导致大鼠抗氧化能力下降及血脂升高,亚麻酸复合粉能够改善高脂肥胖大鼠的相关症状和指标。更多还原

【Abstract】 Objective To evaluate the effect of linolenic acid compound powder(flaxseed microcapsule powder,okra powder, mulberry leaf powder) on the reproductive function of male rats induced by high fat diet. Methods Ninety 8-week-old male SD rats were randomly divided into six groups, with 15 rats in each group. The blank control group(CON group) was fed with normal diet, the model group(HFD group), the low-dose, medium-dose and highdose flaxseed acid compound powder groups(LLA/MLA/HLA groups) and the Simvastatin positive control group(PC group) were fed with high-fat diet. After 4 weeks of high-fat diet modeling, the compound powder was continuously administered by gavage for 4 weeks. The body weight, epididymis and testis weight of rats in each group were observed and recorded. Sperm concentration and motility were detected. Lipid and oxidative stress were also detected. Results Compared with the HFD group, the testis and epididymis weights of rats in each dose group of flaxseed acid compound powder were increased. Compared with the HFD group, the sperm motility and viability in each dose group of flaxseed acid compound powder and the PC group were increased, and the sperm mortality rate was decreased, with statistically significant differences(all P < 0.05). Flaxseed acid compound powder intervention could improve the antioxidant capacity and improve the sex hormone levels of rats. Conclusion A highfat diet leads to a decline in the physiological structure and antioxidant capacity of the testes in rats, as well as changes in lipid levels. Linoleic acid complex powder can improve the related symptoms and indicators in obese rats with high-fat diet.更多还原