【作者】 刘润泽； 范伟； 范兵；

【Author】 LIU Run-ze;FAN Wei;FAN Bing;Wuhan university State Key Laboratory of Oral &Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University;

【机构】 口颌系统重建与再生全国重点实验室,口腔生物医学教育部重点实验室,口腔医学湖北省重点实验室,武汉大学口腔医学院；

【摘要】 目的 :评估二甲双胍对粪肠球菌抵抗氢氧化钙的影响。方法:从通过评估粪肠球菌的存活率、膜电位和膜渗透性、细胞内pH和ATP以及膜转运相关基因的表达,验证了二甲双胍对粪肠球菌抵抗氢氧化钙能力的影响。此外,还测试了二甲双胍对氢氧化钙清除牙本质表面的粪肠球菌生物膜能力的影响。在大鼠根尖周炎模型中,进一步研究了联合应用二甲双胍和氢氧化钙在体内治疗效果。结果:二甲双胍降低了粪肠球菌对氢氧化钙的碱抗性,同时还增强了氢氧化钙对牙本质片上粪肠球菌生物膜的抗菌特性。二甲双胍和氢氧化钙联合应用能进一步促进对大鼠根管内感染的控制和根尖周病变的愈合。结论:二甲双胍可以显著降低粪肠球菌对氢氧化钙的碱抗性,在体外和体内均可以提高氢氧化钙对粪肠球菌的抗菌效果。更多还原

【Abstract】 Objective: To evaluate the effect of metformin(Met) on the alkaline resistance of Enterococcus faecalis(E. faecalis) to calcium hydroxide(CH). Methods:The effect of Met on CH resistance of E. faecalis was verified by evaluating the survival, membrane potential and permeability, intracellular pH and ATP, and the expression of membrane transporter-related genes of E. faecalis. The effect of Met on the ability of CH to remove E. faecalis biofilm on the dentin surface was also tested.The in vivo therapeutic effect of Met plus CH(CHM) was further investigated in a rat apical periodontitis model induced by E. faecalis. Results: Met reduced the alkaline resistance of E. faecalis in the presence of CH, while also enhancing the antimicrobial properties of CH against E. faecalis biofilm on dentin. CHM showed the synergistically promoted intra-canal E. faecalis infection control and healing of periapical lesion in rats. Conclusion: Met could significantly reduce the alkaline resistance of E. faecalis against CH, and improved the antibacterial effect of CH against E. faecalis infection both in vitro and in vivo.更多还原