Comparative investigation on metabolites and biological activities of Huperzia Serrata from different geographical regions

【作者】 赵晨净； 曹朵； 郭斌； 尉亚辉；

【机构】 延安大学； 西北大学；

【摘要】 Huperzia Serrata(H.serrata),being native to south China,is widely distributed in Hubei,Guangxi,Shaanxi,Yunnan,Zhejiang,Hunan,Guizhou and Fujian provinces and Chongqing city.However,the quality of medicinal materials growing in different origins varies greatly,and cannot meet the market demand for high-quality H.serrata.In this study,1061 metabolites were identified,and 54 significantly different secondary metabolites of H.serrata make different origin areas clearly separated(total 116 secondary compounds of H.serrata were indentified).Our results suggested that the medicinal differences of H.serrata from different origins can be elucidated via the variations in the abundance of the alkaloid.We found that huperzine A and the total alkaloids content in Qinling(QL)and Hanzhong(HZ) of Shaanxi province were higher than those in Chongqing(CQ),Hubei(HB),Guangxi(GX),Yunnan(YN),Zhejiang(ZJ),Hunan(HN),Guizhou(GZ) and Fujian(FJ),suggesting that Shaanxi province can be considered as one of the areas that produce high-quality medicinal materials.Additionally,huperzine T,huperzine V,serretezomine A,N-methylycoding,serratezomine E,lycoposerromine U could be used as ideal marker compounds for the quality control of H.serrata from different origins caused by metabolites,and the H.serrata planted in Shaanxi province is well worth exploiting.更多还原

【Abstract】 Huperzia Serrata(H.serrata),being native to south China,is widely distributed in Hubei,Guangxi,Shaanxi,Yunnan,Zhejiang,Hunan,Guizhou and Fujian provinces and Chongqing city.However,the quality of medicinal materials growing in different origins varies greatly,and cannot meet the market demand for high-quality H.serrata.In this study,1061 metabolites were identified,and 54 significantly different secondary metabolites of H.serrata make different origin areas clearly separated(total 116 secondary compounds of H.serrata were indentified).Our results suggested that the medicinal differences of H.serrata from different origins can be elucidated via the variations in the abundance of the alkaloid.We found that huperzine A and the total alkaloids content in Qinling(QL)and Hanzhong(HZ) of Shaanxi province were higher than those in Chongqing(CQ),Hubei(HB),Guangxi(GX),Yunnan(YN),Zhejiang(ZJ),Hunan(HN),Guizhou(GZ) and Fujian(FJ),suggesting that Shaanxi province can be considered as one of the areas that produce high-quality medicinal materials.Additionally,huperzine T,huperzine V,serretezomine A,N-methylycoding,serratezomine E,lycoposerromine U could be used as ideal marker compounds for the quality control of H.serrata from different origins caused by metabolites,and the H.serrata planted in Shaanxi province is well worth exploiting.更多还原