【作者】 孙翊； 张永春； 殷丽青； 杨柳燕； 李心； 李青竹；

【Author】 SUN Yi;ZHANG Yong-chun;YIN Li-qing;YANG Liu-yan;LI Xin;LI Qing-zhu;Forestry and Pomology Research Institute, Shanghai Academy of Agricultural Sciences;Shanghai Key Laboratory of Protected Horticulture Technology;

【机构】 上海市农业科学院林木果树研究所； 上海市设施园艺技术重点实验室；

【摘要】 利用SRAP分子标记技术对97个朱顶红(Hippeastrum spp.)品种进行遗传多样性分析,试验结果显示,9对SRAP引物能够扩增获得473个位点,平均每对引物得到52.6个位点,其中456个为多态性位点,多态性比例为96.41%。说明在朱顶红品种间遗传差异大,分子水平上的遗传多样性十分丰富。其中4对引物组合对供试97个品种的鉴别能力均能够达到100%。聚类分析结果表明,97个品种间遗传相似性系数在0.59-0.91之间,在遗传相似性系数为0.68处分为7个类群,第Ⅰ类群以红色单瓣品种为主,第Ⅱ类群以橙色或粉色单瓣品种为主。白色品种主要分布在第Ⅰ类群,较为稀少的绿色品种分布在第Ⅱ类群和第Ⅳ类群。绝大部分类群中均有重瓣品种分布,其在每个类群中的分布相对集中。‘641’和‘641粉’的遗传相似性系数最高,与仅在花色性状上有表型差异相符。本研究为朱顶红品种鉴定和亲缘关系分析等方面研究的提供重要依据,也能够为品种保存、利用以及种质创新等工作奠定良好基础。更多还原

【Abstract】 The genetic diversity of 97 germplasm of Hippeastrum spp. was studied by sequence related amplified polymorphism(SRAP) molecular marker technique. Results showed that 473 loci of which 456 were polymorphic were amplified by 9 pairs of SRAP primers. On average, each pair of primers produced 52.6 loci. The percentage of polymorphic loci was 96.41%.A high level of genetic variation exists in H. spp. germplasm examined, and extensive genetic diversity was presented. The identification ability for 97 germplasm examined of bands amplified by four primers reaches to 100%. The cluster analysis result shows that the genetic similarity coefficient of 97 germplasms of H. spp. ranged from 0.59 to 0.91, and these germplasm can be divided into 7 groups at the genetic similarity coefficient of 0.68.GroupⅠwas based on single red flower cultivars, and groupⅡwas based on single orange or pink flower cultivars. White flower cultivars mainly distributed among groupⅠ, precious green flower cultivars belong to groupⅡ or groupⅣ. Double flower cultivars distributed into the vast majority of groups, while these cultivars were relatively centralized in each group. The highest genetic similarity coefficient was between ‘641’ and ‘641 pink’ conforming to they only had different in flower color phenotype. This study provided important basis for variety identification and genetic relationship analysis of H. spp., and also established a good foundation for their conservation, utilization and innovation in the future.更多还原