【作者】 张帅； 许淑鱼； 刘欣媛； 李蔚娴； 孙国杰； 田青； 杜艳军；

【Author】 ZHANG Shuai;XU Shu-yu;LIU Xin-yuan;LI Wei-xian;SUN Guo-jie;Tian Qing;DU Yan-jun;College of Acupuncture-Moxibustion and Orthopedics,Hubei University of Chinese Medicine;Tongji Medical College,Huazhong University of Science and Technology;Hubei International Science and Technology Cooperation Base of Preventive Treatment by Acupuncture and Moxibustion;Hubei Shizhen Laboratory;

【机构】 湖北中医药大学针灸骨伤学院； 华中科技大学同济医学院； 湖北时珍实验室； 针灸治未病湖北省国际科技合作基地；

【摘要】 目的:利用功能性超声(functional Ultrasound,f US)技术探讨相同时辰电针不同穴位即"同时不同穴"的脑功能效应。方法:将9只C57BL/6小鼠随机分为合谷穴组、太冲穴组、足三里穴组,每组3只,双侧穴位均给予相同的电针刺激模式(2Hz)。采用f US收集电针前(Pre)、电针中(Acu)的图像数据和脑血流信号的动态改变。脑功能连接分析以海马区(Hippocampal region,HIP)作为感兴趣区域(Region of Interest,ROI)和其他各区域功能连接的改变。结果:合谷穴组脑区激活面积和激活信号强度均高于其他两组,并有多个脑区出现高信号反应,以海马区(HIP)和大脑皮层中后顶叶联合区(PTLp)、压后皮层(RSC)为主。脑血流动力学分析中,合谷穴组和足三里穴组均出现了与电针刺激模式相符合的针刺应答效应,其中合谷穴组针刺应答效应更佳,且海马区响应信号增幅更明显;电针前、中以海马区为基准的脑功能连接相关性动态分析,合谷穴组在电针任务态时涉及脑区更多,包括单侧以及双侧脑区之间交互关系的调节显著,并集中在压后皮层(Retrosplenial cortex)、体感区(Somatosensory area,SS)、下丘脑(Hypothalamus)和嗅球区(Olfactory area,OLF),其中双侧海马区之间功能连接改变更为显著。结论:卯时电针合谷穴组的针刺脑效应优于他时他经穴位。说明合谷穴作为卯时正经时的应时穴更易被激发应时经和应时脏腑的能量场,为佐证"侯气逢时针刺"与子午流注针法的有效性的提供有力依据。更多还原

【Abstract】 Objective:To explore the brain functional effects of electroacupuncture(EA) at different acupoints at the same time about Maoshi(05:00-07:00)by functional ultrasound(fUS).Methods:Nine C57BL/6 mice were randomly divided into Hegu(LI 4) group,Taichong(LV 3) group and Zusanli(ST 36) group,each with 3 mics.All mice were given the same EA(2 Hz) stimulation mode with bilateral acupoints.f US was used to collect image data and dynamic changes of cerebral blood flow signals before and during the electroacupuncture.Brain functional connectivity was analyzed by using hippocampal region(HIP) as ROI and changes in functional connectivity of other regions.Results:The activation area and activation signal intensity of Hegu(LI 4) group were higher than those of the other two groups,and there were high signal responses in several brain regions,mainly in hippocampal region(HIP),middle and posterior parietal lobe(PTLp) and retrosplenial cortex(RSC).In the analysis of cerebral hemodynamics,the acupuncture response effect consistent with the EA stimulation pattern appeared in both Hegu(LI 4) and Zusanli(ST 36) groups,but the acupuncture response effect of Hegu(LI 4) group was better,and the response signal amplitude of HIP was more obvious.Dynamic analysis of brain functional connectivity correlation based on HIP before and during EA showed that more brain regions were involved in the Hegu(LI 4) group during EA task state,including significant adjustment of interaction between unilateral and bilateral brain regions,and concentrated in Retrosplenial cortex(RSC) and Somatosensory area(SS),Hypothalamus(HY),and Olfactory area(OLF),with more significant changes in functional connections between bilateral HIP.Conclusion:The brain efficacy of Maoshi EA on the Hegu(LI 4) group is superior to that of other meridian points with different time.This indicates a heightened susceptibility of the Hegu(LI 4) to stimulation by the energy field of the meridian and zang-fu organs because of the closely corresponding relationship.This provides substantial evidence for the effectiveness of acupuncture on Hou Qi Feng Shi and the Ziwu Liuzhu acupuncture.更多还原