【作者】 陆方； 杨烁慧； 詹松华； 张成； 韩志宏；

【Author】 Lu Fang;Yang Shuo-hui;Zhan Song-hua;Zhang cheng;Han Zhi-hong;Department of Radiology,Shanghai University of Traditional Chinese Medicine Affiliated Shuguang Hospital;Department of Radiology,Shanghai Municipal Hospital of Traditional Chinese Medicine;Siemens Healthineers (China) Co.,Ltd.;Department of Pathology,Shanghai University of Traditional Chinese Medicine Affiliated Shuguang Hospital;

【机构】 上海中医药大学附属曙光医院放射科； 上海市中医药大学附属上海市中医医院放射科； 西门子医疗(中国)有限公司； 上海中医药大学附属曙光医院病理科；

【摘要】 目的:应用超微超顺磁性氧化铁(USPIO)动态磁敏感加权增强(DSC) MRI评估针刺对永久性大脑中动脉栓塞(pMCAO)大鼠亚急性期缺血脑区侧枝循环建立的有效性。方法:建立36只SD大鼠pMCAO模型和假手术组模型:假手术组9只(A组)、无针刺对照组13只(B组)、电针组14只(C组)。每组再分为72h、5天、7天3个亚组。每只大鼠均在相应时间点在1.5T MRI行USPIO-DSC MRI扫描。测量模型鼠缺血区域的相对脑血容量(r CBV)、相对脑血流量(r CBF)、平均通过时间(MTT)和达峰时间(TTP),获得对侧镜像区域参数值,计算比值(rrCBV、rrCBF、rMTT和rTTTp)。观察各个时间点各组间的各参数比值有无统计学差异,评估B组加C组模型鼠rr CBV、rr CBF值与抗CD31染色阳性血管面积百分比(%VA)、微血管计数(MVD)以及血清血管内皮生长因子(VEGF)是否相关。结果:C组5天和7天亚组大鼠的rr CBV分别明显高于B组(P<0.05);C组5天亚组的rr TTP明显低于B组(P<0.05),B组加C组p MCAO模型鼠5天、7天的rr CBV和rr CBF分别与%VA、MVD以及VEGF显著正相关(P<0.05)。结论:通过USPIO-DSC MRI,发现针刺通过促进缺血脑区侧枝循环微血管数目和容量的增加来改善永久性大鼠局灶性脑缺血亚急性期缺血脑区的血供。更多还原

【Abstract】 Objective To evaluate the effectiveness of acupuncture in establishing collateral circulation in subacute ischemic brain regions of rats with permanent middle cerebral artery occlusion(pMCAO) using ultra superparamagnetic iron oxide(USPIO) dynamic magnetic sensitivity weighted enhancement(DSC) MRI.Methods p MCAO model and sham operation group model were established in 36 SD rats:9 rats in sham operation group(group A),13 rats in control group without acupuncture(group B),and 14 rats in electroacupuncture group(Group C).Each group was further divided into three subgroups:72h,5 days and 7 days.Each rat underwent uspio-dsc MRI scanning at 1.5T MRI at the corresponding time point.The relative cerebral blood volume(rCBV),relative cerebral blood flow(rCBF),mean transit time(MTT) and time to peak(TTP) in the ischemic area of the model rats were measured,and the parameter values of the contralateral mirror area were obtained,and the ratios(rrcbv,rrcbf,rmtt and rtttp) were calculated.The ratio of each parameter between each group at each time point was observed for statistical differences,and the correlation between the rrcbv and rrcbf values and the percentage of anti-CD31 staining positive vessel area(% va),microvessel count(MVD) and serum vascular endothelial growth factor(VEGF) of model mice in group B plus group C was evaluated.Results the rrcbv of 5-day and 7-day subgroups in group C was significantly higher than that in group B(P<0.05);The rrttp of the 5-day subgroup in group C was significantly lower than that in group B(P<0.05).The rrcbv and rrcbf of the 5-day and 7-day pMCAO model mice in group B plus group C were significantly positively correlated with% va,MVD and VEGF,respectively(P<0.05).Conclusion through uspio-dsc MRI,acupuncture can improve the blood supply of the ischemic brain region in the subacute phase of permanent focal cerebral ischemia in rats by promoting the increase of the number and volume of collateral circulation microvessels in the ischemic brain region.更多还原