【作者】 张嘉蓉； 王昊； 王子鹤； 张波； 李慧静； 孙倩倩；

【Author】 Zhang Jiarong;Wang Hao;Wang Zihe;Zhang bo;Li Huijing;Sun Qianqian;Institute of Food Science and Technology,CAAS/Comprehensive Utilization Laboratory of Cereal and Oil Processing,Ministry of Agriculture and Rural Affairs;College of Food Science and Technology,Hebei Agricultural University;Zhongyuan Research Center,Chinese Academy of Agricultural Sciences;Institute of Food Science Technology Nutrition and Health (Cangzhou) CAAS;

【机构】 中国农业科学院农产品加工研究所/谷类和油加工综合利用实验室； 河北农业大学食品科技学院； 中国农业科学院中原研究中心； 中国农业科学院农产品加工与营养健康研究院(沧州)；

【摘要】 本研究采用气相色谱-质谱联用技术(HS-SPME-GC-MS)对两个盐碱度不同地区的5个小麦品种进行了挥发性化合物组成及含量分析,共筛选出76种挥发性化合物。配对T检验结果显示,柠檬烯、薰衣草内酯、β-蒎烯、1,6-二甲基萘以及1,8-二甲基萘的含量显著升高(P <0.05),而丁酸和反,反-3,5-辛二烯-2-酮的含量显著降低(P <0.05);同时多因素方差分析表明,这七种化合物在受到种植地因素影响较大,而品种因素影响较小,并且种植地土壤盐碱程度因素贡献率可达70%以上。通过计算这7种化合物的相对气味活性值(ROAV)发现,除了丁酸ROAV小于1外,其余6种挥发性化合物ROAV值均大于1,说明这6种挥发性化合物对盐碱地种植小麦风味贡献较大。通过主成分分析(PCA)和线性判别分析(LDA)的结果验证,柠檬烯、薰衣草内酯、β-蒎烯、1,6-二甲基萘、1,8-二甲基萘、反,反-3,5-辛二烯-2-酮是盐碱地种植小麦的特征挥发性化合物。更多还原

【Abstract】 In this study,headspace solid phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS) was used to analyze the composition and content of 5 wheat varieties in two areas of different salinity,and a total of 76 volatile compounds were selected.Paired T-test showed that the contents of(D)-Limonene,Lavender lactone,beta-Pinene,1,6-dimethyl-Naphthalene and 1,8-dimethyl-Naphthalene increased significantly(P <0.05),while butanoic acid and(E,E)-3,5-Octadien-2-one decreased significantly(P <0.05).At the same time,multifactor analysis of variance showed that these seven compounds were greatly affected by planting site factor,but was less affected by variety factor,and the contribution rate of soil salinity and alkalinity levels of planting site was more than 70%.By calculating the relative odor activity value(ROAV) of these seven compounds,it was found that except for butanoic acid with a ROAV was less than 1,the other six volatile compounds had ROAVs greater than 1,indicating that these six volatile compounds contributed more to wheat aroma in the saline-alkali soil.The results of principal component analysis(PCA) and linear discriminant analysis(LDA) verified that(D)-Limonene,Lavender lactone,beta-Pinene,1,6-dimethyl-Naphthalene and 1,8-dimethyl-Naphthalene and(E,E)-3,5-Octadien-2-one are characteristic volatile compounds of wheat cultivated in the saline-alkali soil.更多还原