【作者】 肖鹏飞； 吴韵雅； 邹桂伟； 梁宏伟；

【Author】 XIAO Peng-Fei;WU Yun-Ya;ZOU Gui-Wei;LIANG Hong-Wei;College of Fisheries and Life Science,Shanghai Ocean University;Yangtze River Fisheries Research Institute,Chinese Academy of Fisheries Science;

【机构】 上海海洋大学水产与生命科学学院； 中国水产科学研究院长江水产研究所；

【摘要】 为了从生长性能,肌肉营养价值和营养代谢方面探究两种鲤颖鲤和散鳞镜鲤对高糖饲料的适应和利用能力,以初始体质量(5.01±0.2)g的颖鲤和(4.5±0.2)散鳞镜鲤为研究对象,分别配置配制低碳水化合物(LC,20%C),普通碳水化合物(NC,30%C)和高碳水化合物(HC,40%C)的等氮饲料分组投喂8周,每组设置3个重复,每个重复30尾鱼。结果显示相较于低糖组(20%)和高糖组(40%),在正常糖(30%)水平下饲养,两种鲤的最终体重、饲料效率和特定生长率等指标均显著升高(P<0.05),促进了生长。在饲料中添加糖显著提高了两种鲤肌肉中的必需氨基酸、呈味氨基酸和总氨基酸含量(P<0.05),但是显著减少了肌肉中的不饱和脂肪酸含量。当饲料中糖水平为20%时,在两种鲤的肌肉中和不饱和脂肪酸合成相关的基因acsbg2,elovl5,lpl-α,elovl6的表达均显著高于其他两个处理组(P<0.05);关于延长多不饱和脂肪酸链的基因elovl5和elovl6,在饲料中糖水平添加为30%时,在颖鲤的肌肉中的表达量显著高于散鳞镜鲤(P<0.05)。两种鲤对于糖的利用来看,散鳞镜鲤对糖的耐受性更强;但是在一定量的糖水平喂养下,颖鲤相较于散鳞镜鲤拥有更好的肌肉营养品质。本研究中,使用添加了20%糖水平的饲料,两种鲤的肌肉品质相比较最好,而颖鲤比起散鳞镜鲤拥有更好的肌肉品质;当饲料中糖水平提高到30%,两种鲤的生长速度最快,而散鳞镜鲤较于颖鲤拥有更好的生长情况。更多还原

【Abstract】 In order to investigate the adaptation and utilisation capacity of two carp species,hybrid of common carp,crucion carp and mirror carp,Ying and Cyprinus carpio var.specularis,to high-carbohydrate diets in terms of growth performance,muscle nutritive value and nutrient metabolism,common carp and common mirror carp with an initial body mass of(5.01±0.2) g were fed isonitrogenous diets of low carbohydrate(LC,20%C),normal carbohydrate(NC,30%C) and high carbohydrate(HC,40%C) in groups for 8 weeks.The isonitrogenous diets of low carbohydrate(LC,20%C),normal carbohydrate(NC,30%C) and high carbohydrate(HC,40%C) were fed in groups for 8weeks,with three replicates of 30 fish in each group.The results showed that compared to the low(20%) and high(40%) carbohydrate groups,feeding at the normal carbohydrate level(30%) promoted growth with significantly higher(P<0.05) final body weight,feed efficiency and specific growth rate in both carp species.Addition of sugar to the diets significantly(P<0.05) increased the content of essential,flavour and total amino acids in the muscle of both carp species,but significantly(P<0.05)decreased the content of unsaturated fatty acids in the muscle.The expression of acsbg2,elovl5,lpl-αand elovl6,genes related to unsaturated fatty acid synthesis,was significantly higher(P<0.05) than that of the other two treatment groups in the muscles of both carp species at 20% dietary carbohydrate level;and the expression of elovl5 and elovl6,genes related to polyunsaturated fatty acid chain elongation,was significantly(P<0.05).The expression of elovl5 and elovl6,genes involved in the elongation of polyunsaturated fatty acid chains,was significantly(P<0.05) higher than that of the other two treatment groups in the muscles of the two carp species at 30% of the dietary carbohydrate level.In terms of carbohydrate utilisation by the two carp species,mirror carp was more tolerant to carbohydrate;however,Ying carp had better muscle nutritional quality compared to mirror carp when fed at a given sugar level.In the present study,the best muscle quality was obtained at 20% carbohydrate,while Ying carp had better muscle quality than mirror carp;the fastest growth was obtained when the carbohydrate level was increased to 30%,while mirror carp had better growth than Ying carp.更多还原