【作者】 刘斌； 黄梓芮；

【Author】 Bin Liu;Zirui Huang;College of Food Science,Fujian Agriculture and Forestry University;National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University;

【机构】 福建农林大学食品科学学院； 国家菌草工程技术研究中心；

【摘要】 桑黄富含多酚类、黄酮、多糖类、萜类等多种生物活性物质。本研究以杨树桑黄子实体为研究目标,分离出三种不同的桑黄活性组分,包括桑黄醇溶物(SVE)、桑黄水溶物(SVW)以及桑黄多糖(SVP)。对SVE、SVW以及SVP的得率、化学组成、体外自由基清除能力以及α-葡萄糖苷酶抑制活性进行分析;通过建立T2DM小鼠模型,研究桑黄活性组分对小鼠T2DM的调节作用和对小鼠肠道菌群的影响。应用代谢组学技术研究了桑黄水溶物干预对T2DM小鼠肠道代谢物的影响,并结合粪菌移植技术深入地探讨了桑黄水溶物干预后的粪菌液对T2DM小鼠的改善作用及其机制。更多还原

【Abstract】 The ’Sanghuang’(Sanghuangporus) is rich in a variety of bioactive substances such as polyphenols,flavonoids,polysaccharides,terpenoids,etc.In this study,three different active ingredients separated from Sanghuangporus vaninii(the specie of the largest domestic market and the most mature cultivation technology of the seeds in China),including Sanghuangporus vaninii ethanol-solubles(SVE),Sanghuangporus vanini water-solubles(SVW),and Sanghuangporus polysaccharides(SVP).In addition,the yield,chemical composition,free radical scavenging ability in vitro,and α-glucosidase inhibitory activity of SVE,SVW,and SVP were analyzed.It was established that a T2DM mouse model to study the regulatory effects of the active components of Sanghuangporus vaninii on the development of T2DM and the effects of gut microbiota in mice.Besides,metabolomics techniques were applied to investigate the effects of SVW intervention on intestinal metabolites in T2DM mice,and the improvement mechanism of fecal solution from SVE intervention mice on T2DM was investigated by FMT technique.更多还原