【作者】 刘润泽； 范伟； 范兵；

【Author】 LIU Run-ze;FAN Wei;FAN Bing;Wuhan university State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration,Key Laboratory of Oral Biomedicine Ministry of Education,Hubei Key Laboratory of Stomatology,School & Hospital of Stomatology,Wuhan University;

【机构】 口颌系统重建与再生全国重点实验室,口腔生物医学教育部重点实验室,口腔医学湖北省重点实验室,武汉大学口腔医学院；

【摘要】 目的:将从根管治疗失败的根管中采集的野生型粪肠球菌的碱抗性与标准菌株进行了比较,进一步探究难治性根尖周炎的根管中野生型粪肠球菌碱抗性的特点。方法:从根管治疗失败患者的根管中分离出野生型菌株(L1, L2, L3, L4, L5, L6),并对其进行鉴定。利用动态生长曲线比较了24小时内在正常BHI(pH=7)和碱性BHI(pH=10)培养中野生型菌株(L1, L2, L3, L4, L5, L6)和标准型菌株(ATCC 29212)的生长差异。结果:在正常BHI(pH=7)中,野生型菌株(L1, L2, L3, L4, L5, L6)和标准型菌株(ATCC29212)的生长速度无显著差异;在碱性BHI(pH=10)中,野生型菌株(L1, L3, L4, L5,L6)的生长速度显著高于标准型菌株(ATCC 29212),野生型菌株(L2)的生长速度低于标准型菌株(ATCC 29212)。结论:来自不同患者的野生型粪肠球菌的碱抗性存在差异。大部分野生型菌株的碱抗性高于标准型粪肠球菌,但仍有少数野生型菌株的碱抗性低于标准型粪肠球菌。更多还原

【Abstract】 Objective:The alkaline resistance of wild-type Enterococcus faecalis(E.faecalis collected from root canals with failed root canal treatment were compared with standard strain to further investigate the characteristics of the alkaline resistance of wild-type E.faecalis in root canals with refractory periapical infections.Methods:Wild-type strains(L1,L2,L3,L4,L5,L6) were isolated from root canals of patients with failed root canal treatment and characterized.The growth differences between the wild-type strains(L1,L2,L3,L4,L5,L6) and the standard strain(ATCC 29212) in normal BHI(pH=7) and alkaline BHI(pH=10) cultures were compared over a period of 24 hours using dynamic growth curves.Results:In normal BHI(pH=7),there was no significant difference in growth rate between the wild-type strains(L1,L2,L3,L4,L5,L6) and the standard strain(ATCC 29212);in alkaline BHI(pH=10),the growth rate of wild-type strains(L1,L3,L4,L5,L6) was significantly higher than that of the standard strain(ATCC 29212).The growth rate of the wild-type strain(L2) was lower than that of the standard strain(ATCC 29212).Conclusion:The alkaline-resistance of wild-type E.faecalis from different patients varies.Most of the wild-type strains were more alkaline-resistant than standard E.faecalis,but there were still wild-type strains with lower alkaline resistance than standard E.faecalis.更多还原