【作者】 陈倩洋； 郭水香； 张格格； 王丽娜；

【Author】 Chen Qianyang;Guo Shuixiang;Zhang Gege;Wang Lina;Department of Endodontics,Dalian Medical University;

【机构】 大连医科大学口腔医学院；

【摘要】 目的:牙周炎是牙周支持组织的炎症性、破坏性疾病。中药作为牙周病治疗中调节宿主免疫反应的一个辅助方法,有待于进一步的研究和发掘。薯蓣(Dioscorea opposita)又称山药(YAM),本研究以薯蓣为前驱体,采用水热合成法合成了碳点来探讨薯蓣碳点(YAM-CDs)治疗牙周炎的作用研究。方法:采用透射电子显微镜、傅立叶变换红外光谱、紫外光谱、荧光光谱和X射线光电子能谱对材料进行了表征检测,并建立YAM-CDs作用于LPS介导下的MC3T3-E1细胞炎症微环境的体外模型,通过Real-time qPCR、Western Blot,研究YAM-CDs对MC3T3-E1细胞增殖分化的作用。结果:薯蓣碳点为球形结构,表面含有大量活性基团,颗粒尺寸在2-5 nm之间。Real-time qPCR结果显示,YAM-CDs组ALP、RUNX2基因水平表达较空白对照组升高(P<0.05),LPS组ALP、RUNX2基因水平表达较空白对照组降低(P<0.05),LPS+YAM-CDs组ALP、RUNX2基因水平表达较LPS组升高(P<0.05)。Western Blot结果显示趋势同Real-time qPCR趋势相一致。结论:薯蓣碳点通过促进成骨相关因子的表达促进成骨细胞增殖分化,具有明显的治疗牙周炎的作用,为研究薯蓣的有效物质基础提供了新的思路。更多还原

【Abstract】 OBJECTIVE:Periodontitis is an inflammatory,destructive disease of the periodontal supporting tissues.Herbal medicine as an adjunct to modulating the host immune response in the treatment of periodontal disease needs to be further researched and explored.Dioscorea opposita also known as yam(YAM).In the present study,carbon dots were synthesized by hydrothermal synthesis to investigate the role of Dioscorea carbonica dots(YAM-CDs) in the treatment of periodontitis using Dioscorea as a precursor.METHODS:Transmission electron microscopy,Fourier transform infrared(FTIR)spectroscopy,ultraviolet(UV) spectroscopy,fluorescence spectroscopy and X-ray photoelectron spectroscopy were used to characterize the materials and to establish an in vitro model of the action of YAM-CDs on the LPS-mediated inflammatory microenvironment of MC3T3-E1 cells.The role of YAM-CDs on the proliferation and differentiation of MC3T3-E1 cells was investigated by Real-time qPCR and Western Blot.RESULTS:Dioscorea carbon dots have a spherical structure with a large number of reactive groups on the surface and a particle size between 2-5 nm.Real-time qPCR results showed that the expression of ALP and RUNX2 gene levels in the YAM-CDs group was higher than that in the blank control group(P<0.05),the expression of ALP and RUNX2 gene levels in the LPS group was lower than that in the blank control group(P<0.05),and the expression of ALP and RUNX2 gene levels in the LPS+YAM-CDs group was higher than that in the LPS group(P<0.05).Western Blot results showed a trend consistent with that of Real-time qPCR.CONCLUSION:Dioscorea carbonica dots promote the proliferation and differentiation of osteoblasts by promoting the expression of osteogenesis-related factors,and have obvious therapeutic effects on periodontitis,which provides a new idea for the study of the effective material basis of Dioscorea.更多还原