【作者】 赵裕蓉； 王雅琴； 周莉； 贺群； 莫锡荣； 黎高翔；

【Author】 Zhao Yurong;Wang Yaqing;Zhou Li;He Qun;Mo Xirong;Li Gaoxiang;Beijing Institute of Chemical Technology,Department of Chemical Engeneering;Institute of Microbile,Chinese Academy of Sciences;

【机构】 北京化工学院化学工程系； 中国科学院微生物研究所；

【摘要】 本文主要对用于测定葡萄糖的石墨片介体电极进行了研究讨论。介体二茂铁(Fc)以物理吸附的方式固定于基底石墨片电极上,葡萄糖氧化酶和辣根过氧化物酶以戊二醛共价交联于基底电极表面。电极稳态响应时间30秒。线性范围为0-20mM。本文还讨论了各种动力学因素对电极的影响。阐明了双酶修饰电极在灵敏度和稳定性上均优于单酶电极.更多还原

【Abstract】 The redox-mediated graphite electrode which detect glucose is described.Ferrocene as a mediater is physically absorbed on it.Glucose Oxidase(GOD) and Horsradish Oxidase(HRP) is covelently immobilized with glutaraldehyde(GA)on the base electrode.It reaches a steady state within 30 s.The linear range is 0-20 mM.The influence of several kinetic parameters on the electrode response has been investigated.The electrode which bienzyme modified is better In sensitivity and stability than that one mono-enzyme modified.更多还原