【作者】 庄明亮； 李剑飞； 李志勇； 王志； 葛蓬；

【Author】 Zhuang Mingliang;Li Jianfei;Li Zhiyong;Wang Zhi;Ge Peng;Jilin Provincial Institute of Beekeeping Science;

【机构】 吉林省养蜂科学研究所；

【摘要】 冷冻保存生物材料根据降温速率的不同分为逐步降温冷冻法和玻璃化冷冻法,超低温冷冻蜜蜂精液常采用逐步冷冻法,超低温冷冻蜜蜂卵常采用玻璃化冷冻法。采用逐步降温冷冻法,既可以使用渗透性保护剂又可以使用非渗透性保护剂,利用程序降温仪,精确实现以2～5℃/min速率降温至-40℃,再投入到液氮中保存。采用玻璃化冷冻法,要经过渗透化处理去除卵壳和蜡质,使用高浓度的可渗透性保护剂,使卵快速达到液氮温度,迅速通过细胞冰晶形成的临界温度,从而减轻冷冻损伤。更多还原

【Abstract】 Cryopreserved biomaterials can be divided into stepwise cooling and vitrification according to the different cooling rates.Cryopreservation of bee semen often adopts stepwise freezing,and cryopreservation of bee embryos often adopts vitrification.The gradual cooling and freezing method can be used to use both permeable and non-permeable protective agents.The programmed cooling instrument can be used to accurately reduce the temperature to-40℃ at the rate of 2～5℃/min,and then put into liquid nitrogen for storage.The vitrification method is used to remove the eggshell and wax through osmotic treatment,and use a high concentration of permeable protective agent to make the embryo quickly reach the liquid nitrogen temperature and quickly pass the critical temperature of cell ice crystal formation,so as to reduce the freezing damage.更多还原