【作者】 杨少华； 毕晓君； 谢岩； 张毅； 张胜利； 张勤； 孙东晓；

【Author】 Shaohua Yang;Xiaojun Bi;Yan Xie;Yi Zhang;Shengli Zhang;Qin Zhang;Dongxiao Sun;Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University;

【机构】 中国农业大学动物科技学院农业部动物遗传育种与繁殖重点实验室畜禽育种国家工程实验室；

【摘要】 基于课题组前期产奶性状全基因组关联分析(GWAS)结果,本研究将PDE9A基因作为产奶性状候选关键基因,进一步通过SNaPshot分型技术,分析了PDE9A基因启动子区在8个公牛家系的506头中国荷斯坦牛女儿群体中的多态性及其对5个产奶性状的遗传效应。采用混合动物模型进行最小二乘法拟合线性模型分析,关联分析结果表明:c.-1376 G>A和c.-724A>G均与产奶量(P<0.0001; P<0.0001),乳脂量(P=0.0009; P=0.0016)和乳蛋白量(P<0.0001; P<0.0001)显著相关。单倍型分析结果与单个SNP分析结果一致。RT-PCR结果表明PDE9A基因在乳腺组织中特异性高表达。以上结果显示PDE9A为影响产奶性状的关键基因,可进一步开展功能验证。更多还原

【Abstract】 Based on our previous genome-wide association analysis on milk production traits in Chinese Holstein, the PDE9A gene was considered as a key candidate for milk production traits. Using SNaPshot assay, two SNPs in 5’regulatory region were chosen for genotyping in 506 Chinese Holstein cows and identification of genetic effects on milk traits. With a mixed animal model, we found that c.-1376 G>A and c.-724A>G were significantly associated with milk yield, fat yield and protein yield(P=0.0016～<0.0001). Haplotype analysis was consistent with the results of the single SNP. In addition,PDE9A was highly expressed in mammary gland of lactating cows compared with other 7 tissues using RT-PCR method(p<0.01). Our findings indicated that the PDE9A gene is a key gene with significant genetic effect for milk traits and needed for further function validation.更多还原