【作者】 刘换换； 王斐； 胡月苗； 何国仁； 胡凤荣；

【Author】 LIU Huan-huan;WANG Fei;HU Yue-miao;HE Guo-ren;HU Feng-rong;College of Landscape Architecture,Nanjing Forestry University;

【机构】 南京林业大学风景园林学院；

【摘要】 以1M HCL、45%HAc、0.5%纤维素酶和果胶酶的混合液三种试剂作为解离液,按不同方式组合后,对经过预处理和卡诺氏固定液I固定后的风信子根尖按不同的时间梯度进行解离、压片、观察。结果表明:5种解离方法均可以获得分散良好、形态正常、易于计数和进行核型分析的染色体制片。试验中观察到HAc能有效去除根尖细胞的细胞壁,并能分解细胞内抑制染色体分散的物质。在后续染色体制片中,可考虑优先选用45%HAc作为解离试剂。更多还原

【Abstract】 This study used three kinds of reagents as isolation solution in different ways, including 1M HCL, 45% HAc and 0.5% cellulase and pectinase mixture, separated, pressed and observed at different time gradient after handling with pretreatment and Carnoy’s Fluid I of Hyacinthus root tip. The results showed that the 5 ways of isolating could obtain good results of the chromosome, which were well dispersed, normal and easy to count and karyotype analysis. It was observed that HAc could remove effectively the cell wall of root tip cells and break down the substances that inhibit chromosome dispersion. In the subsequent chromosome squashing, 45% HAc can be given priority to consider as dissociation reagent.更多还原