【作者】 胡凤荣； 席梦利； 池坚； 施季森；

【Author】 Hu Fengrong;XI Mengli;CHI Jian;SHI Jisen;The Key Laboratory of Forest Tree Genetics and Gene Engineering Nanjing Forestry University;

【机构】 国家林业局林木遗传和基因工程重点实验室(南京林业大学)；

【摘要】 以东方百合栽培品种’Sorbonne’、’Siberia’、’Tiber’的试管苗鳞片和叶片为外植体,研究了6-BA0.3～2.0 mg/L、KT0.5～2.0mg/L、NAA0.1～2.0mg/L、2,4D0.5～2.0mg/L、ZT0.1～0.5 mg/L器官发生的影响;以Tiber’的愈伤组织为外植体探讨了6-BA0.2～1.5 mg/L、2,4-D0.5～2.0 mg/L对体胚发生的影响;以三种东方百合的试管苗为材料,研究了激素与蔗糖浓度对瓶内小鳞茎增重的影响。结果表明:高浓度的激素有利于叶片分化不定芽,6-BA能提高鳞片上不定芽的分化频率,2,4-D能诱导’Tiber’的愈伤组织形成体细胞胚,高蔗糖浓度对瓶内小鳞茎增重起重要作用。更多还原

【Abstract】 With the bulb scale and the leaf of commercial lily varieties’ Sorbonne’,‘Siberia’ ’Tiber’ as initial explants,the effect factors of 6-BA0.3～2.0 mg/L、KT0.5～2.0 mg/L、NAA0.1～2.0 mg/L、2,4-D0.5～2.0 mg/L、ZT0.1～0.5 mg/L on organogenesis of the lilium Oriental were studied.Using the callus as initial explants,the effect factors 6-BA0.2～1.5 mg/L、2,4-D0.5～2.0 mg/L on somatic embryogenesis were examed.Using plantlet as the material,the effect of auxin and the concentration of sucrose on forming bulbs in the bottle were investigted.The results showed that the auxinn in high concentration as was advantageous to adventitious buds differentiation from leaf.6-BA could raise the bud differentiation frequency from the bulb scale.Somatic embryogenesis could be induced from callus by 2,4-D.The sucrose in high concentration played an important role in forming bulblets under tissue culture condition.更多还原