【作者】 张克； 赵祥云； 王文和； 路苹； 薛敦孟； 耿哲；

【Author】 Zhang Ke;Zhao Xiangyun;Wang Wenhe;Lu Ping;Xue Dunmeng;Geng Zhe;Beijing Agricultural College;Beijing Rainbow Flower Bulb Co.,Ltd.;

【机构】 北京农学院园林系； 北京润博球根花卉有限公司；

【摘要】 我们以山丹(Lilium pumilum)、渥丹(L.concolor)为父本,以亚洲百合系白天使(Navona)品种为母本进行杂交,对杂交胚进行抢救获得杂交后代,并将杂交后代进行继代组织培养。提取亲本及杂交后代的总DNA,再选用不同引物,利用RAPD技术对亲本及杂交后代进行分析,判断杂交后代成功与否。研究不同条件对RAPD效果的影响,寻求最佳的分析方案。初步研究结果:选用的五种引物都能扩增出条带,比较亲本和其杂交后代的带谱看出:山丹与白天使的杂交后代有可能为真杂交;而渥丹与白天使的杂交后代不能判断是否为真杂交。更多还原

【Abstract】 The hybrid progenies of Lilium pumilum × "navona" and L.concolor× "navona" were used in this study.We did subculture on the hybrid progenies,then extracted the DNA of them and their parents.We chose different random primer to enlarge those materials and concluded that if it was successful by RAPD analyzing to the enlarged product.At the same time we studied the impact of different conditions to the effect of RAPD analyze.The results were as follows:there was all primer can enlarge out different DNA string in the hybrid progenies and their parents.The hybrid progenies of L.pumilum × "navona" was successful and the other hybrid progenies was unsuccessful by comparing the DNA string of samples.更多还原