【作者】 朱康； 邹回； 陈静； 胡佳琪； 熊勇华； 黄小林；

【Author】 Zhu Kang;Zou Hui;Chen Jing;Hu Jiaqi;Xiong Yonghua;Huang Xiaolin;State Key Laboratory of Food Science and Resources,Nanchang University;School of Food Science and Technology,Nanchang University;

【机构】 南昌大学食品科学与资源挖掘全国重点实验室； 南昌大学食品学院；

【摘要】 乳铁蛋白是转铁蛋白家族中的一种具有生物活性的多功能糖蛋白。作为一种重要的营养强化剂,乳铁蛋白是奶粉中的明星营养素之一。因此,快速准确地定量检测奶粉中的乳铁蛋白含量对评价奶粉质量至关重要。然而,目前报道的大多数乳铁蛋白定量免疫分析方法存在样品前处理繁琐复杂或检测时间过长的问题,不能满足奶粉中乳铁蛋白快速检测的要求。在此,我们报道了一种结合动态光散射(DLS)免疫传感和硼酸盐亲和识别的新方法,用于快速灵敏地检测乳铁蛋白。由于硼酸配体可以选择性识别并结合糖蛋白中的顺式二醇结构,所开发的DLS免疫传感器可以在节省一个抗体的情况下定量检测乳铁蛋白,有效解决了基于夹心法检测生物大分子方法中必要的对配对抗体的需求。此外,DLS免疫传感器对乳铁蛋白表现出优异的特异性和敏感性,将磁性纳米颗粒辅助的免疫磁分离和DLS信号放大策略相结合,该方法可在约15分钟内对乳铁蛋白实现低至1 ng/mL的高灵敏检测。更多还原

【Abstract】 Lactoferrin(Lf) is a bioactive multifunctional glycoprotein that belongs to the transferrin family.As an important nutritional fortifier,Lf is one of the star nutrients in milk powder.Thus,the rapid and accurate quantitative detection of Lf content in milk powder is crucial to the evaluation of its quality.However,most reported immunoassay methods for Lf quantitation suffer from cumbersome sample pretreatment or lengthy testing time and do not meet the requirements for rapid Lf detection in milk powder.Herein,we report a novel method combining dynamic light scattering(DLS) immunosensing and boronate affinity recognition for the rapid and sensitive detection of Lf.Owing to the selective binding between a boronate ligand and the cis diol,the developed DLS immunosensor quantitatively detected Lf with saving one antibody,greatly reducing the need for matching antibodies,which are needed in traditional sandwich immunoassay for biomolecular detection.In addition,the DLS immunosensor exhibited high selectivity and sensitivity to Lf,with a detection limit of 1 ng/mL within～15 min through coupling with magnetic nanoparticle-assisted immunomagnetic separation and DLS signal transduction.更多还原