【作者】 周梦君； 陈樨蕊； 沈轩昂； 林相恺； 陈坪； 李响敏； 熊勇华； 黄小林；

【Author】 Zhou Mengjun;Chen Xirui;Shen Xuanang;Lin Xiangkai;Chen Ping;Li Xiangmin;Xiong Yonghua;Huang Xiaolin;State Key Laboratory of Food Science and Resources,Nanchang University;School of Food Science and Technology,Nanchang University;

【机构】 南昌大学食品科学与资源挖掘全国重点实验室； 南昌大学食品学院；

【摘要】 现有研究表明,使用负载大量发光染料的高亮度荧光微球作为信号探针可显著提高免疫层析分析方法的检测灵敏度。然而,由于聚集引起的荧光淬灭效应,现有荧光微球的光致发光强度有限。为此,本文选用高亮度的红色聚集诱导发射荧光微球(AIENPs)作为免疫层析分析的信号放大探针,用于定量检测玉米赤霉烯酮(ZEN)。在此基础上,系统比较AIENPs和时间分辨荧光纳米微球(TRNPs)的光学性质差异,结果表明AIENPs在硝酸纤维素膜上具有更强的光致发光强度和更好的环境耐受性。此外,在抗体、耗材、试纸条读取设备等相同条件下系统评估了AIENPLFIA与TRNP-LFIA的检测性能,结果表明AIENP-LFIA在0.195～6.25 ng/mL的ZEN浓度范围内表现出优异的动态线性定量关系,其半数抑制浓度和最低检测限分别低至0.78 ng/mL和0.11 ng/mL,相较于TRNP-LFIA分别提高了2.07和2.36倍。此外,准确性、精密度、特异性等评估结果表明,AIENP-LFI可快速、灵敏、准确地实现玉米样本中ZEN的定量检测,具有良好的实用性。更多还原

【Abstract】 Highly luminescent nanospheres have been demonstrated in enhancing the sensitivity of lateral flow immunoassay（LFIA） due to their loading numerous luminescent dyes.However,the photoluminescence intensities of existing luminescent nanospheres are limited due to the aggregation-caused quenching effect.Herein,highly luminescent aggregation-induced emission luminogens embedded nanospheres（AIENPs） with red emission were introduced as signal amplification probes of LFIA for quantitative detection of zearalenone（ZEN）.Optical properties of red-emitted AIENPs were compared with timeresolved dyeembedded nanoparticles（TRNPs）.Results showed that red-emitted AIENPs have stronger photoluminescence intensity on the nitrocellulose membrane and superior environmental tolerance.Additionally,we benchmarked the performance of AIENP-LFIA against TRNP-LFIA using the same set of antibodies,materials,and strip readers.Results showed that AIENP-LFIA exhibits good dynamic linearity with the ZEN concentration from 0.195 to 6.25 ng/mL,with half competitive inhibitory concentration(IC₅₀) and detection of limit（LOD） at 0.78 and 0.11 ng/mL,respectively.The IC₅₀ and LOD are2.07-and 2.36-fold lower than those of TRNP-LFIA.Encouragingly,the precision,accuracy,specificity,practicality,and reliability of this AIENP-LFIA for ZEN quantitation were further characterized.The results verified that the AIENPLFIA has good practicability for the rapid,sensitive,specific,and accurate quantitative detection of ZEN in corn samples.更多还原