Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

【作者】 赵莹； GUAN Zhengzhe； QIN Yanhong； ZHONG Rongrong； ZHANG Yuanyuan； WANG Mengjiao； WEN Caiyi； WANG Fei； 宋露洋； 鲁传涛；

【Author】 ZHAO Ying;GUAN Zhengzhe;QIN Yanhong;ZHONG Rongrong;ZHANG Yuanyuan;WANG Mengjiao;WEN Caiyi;WANG Fei;SONG Luyang;LU Chuantao;College of Plant Protection,Henan Agricultural University;Institute of Plant Protection,Henan Academy of Agricultural Sciences;

【机构】 College of Plant Protection,Henan Agricultural University； Institute of Plant Protection,Henan Academy of Agricultural Sciences；

【摘要】 The root rot of Salvia miltiorrhiza is a common root disease caused by Fusarium spp.,which has become one of the main diseases affecting the production of S.miltiorrhiza.Currently, several hypovirulence-related myeoviruses have been identified in many phytopathogenie fungi including Fusarium spp., which show potential as biological control.This study reports a new mycovirus,Fusarium oxysporum partitivirus 1(FoPV1), isolated from F.oxysporum strain FCR51, a causal agent of S.miltiorrhiza dry rot.The FoPV1 genome contains two double-stranded RNA segments(dsRNAl and dsRNA2).The size of dsRNAl is 1 773 bp, and it encodes a putative RNA-dependent RNA polymerase( RdRp), The dsRNA2 is 1 570 bp in length, encoding a putative capsid protein( CP).Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of RdRp and HP indicated that FoPV1 appears to be a new member of the family Partitiviridae that is related to members of the genus Gammapartitivirus.An assay of pathogenicity showed that FoPV1 confers hypervirulence to its host,F.oxysporum.This is the first report of a partitivirus infecting F.oxysporum and the first hypovirulence-related mycovirus from a causal agent of S.miltiorrhiza dry rot.更多还原

【Abstract】 The root rot of Salvia miltiorrhiza is a common root disease caused by Fusarium spp.,which has become one of the main diseases affecting the production of S.miltiorrhiza.Currently, several hypovirulence-related myeoviruses have been identified in many phytopathogenie fungi including Fusarium spp., which show potential as biological control.This study reports a new mycovirus,Fusarium oxysporum partitivirus 1(FoPV1), isolated from F.oxysporum strain FCR51, a causal agent of S.miltiorrhiza dry rot.The FoPV1 genome contains two double-stranded RNA segments(dsRNAl and dsRNA2).The size of dsRNAl is 1 773 bp, and it encodes a putative RNA-dependent RNA polymerase( RdRp), The dsRNA2 is 1 570 bp in length, encoding a putative capsid protein( CP).Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of RdRp and HP indicated that FoPV1 appears to be a new member of the family Partitiviridae that is related to members of the genus Gammapartitivirus.An assay of pathogenicity showed that FoPV1 confers hypervirulence to its host,F.oxysporum.This is the first report of a partitivirus infecting F.oxysporum and the first hypovirulence-related mycovirus from a causal agent of S.miltiorrhiza dry rot.更多还原