【作者】 潘春柳； 蓝祖栽； 余丽莹； 黄雪彦； 姚李祥； 张占江； 周芸伊；

【Author】 PAN Chunliu;LAN Zuzai;YU Liying;HUANG Xueyan;YAO Lixiang;ZHANG Zhanjiang;ZHOU Yunyi;Guangxi Botanical Garden of Medicinal Plant/Guangxi TCM Resources General Survey and Data Collection Key Laboratory;

【机构】 广西壮族自治区药用植物园/广西中药资源普查与研究重点实验室；

【摘要】 草珊瑚种子具有显著的休眠现象,生产上多采用层积的方式对种子进行休眠破除。为探讨层积处理对草珊瑚种子休眠解除的影响,本文对不同层积处理方式:(20℃层积,4℃层积)和处理时间(0、7、14、24、28d)的草珊瑚种子进行萌发率测定、显微结构观察及生理生化指标测定。结果表明:20℃层积处理使草珊瑚种子发芽率和发芽势更高,更有利于其种胚发育。在20℃和4℃层积条件下,随着层积时间增加,淀粉含量下降,可溶性蛋白和可溶性糖含量先下降后上升,α-淀粉酶活性呈下降趋势;SOD、POD及CAT活性呈先下降后上升趋势;MDA含量呈先上升后下降再上升趋势。说明草珊瑚种子解除休眠过程与种胚发育、贮藏物质变化以及抗氧化酶活性、脂质过氧化水平密切相关。更多还原

【Abstract】 Seeds of Sarcandra glabra have a remarkable dormancy phenomenon, and stratification is often used to break the seed dormancy in production. To study the morphological, physiological and biochemical changes in seed dormancy release of Sarcandra glabra, this study investigated the germination rate, microstructural features and physiological and biochemical indexes of Sarcandra glabra seeds, which were treated with different stratification temperature(20℃, 4℃) at different stratification times(0, 7, 14, 24, 28days). The results showed that seeds with 20℃ stratification had a higher germination rate and germination potential, and more fully developed embryos. Under 20℃and 4℃ stratification condition, with the stratification time increased, the content of starch decreased, the content of soluble protein and soluble sugar decreased first and then increased, the activity of α-amylase decreased, the activity of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) decreased first and then increased, and the content of malondialdehyde(MDA) first increased and then decreased and then increased. These results indicated that the changes in embryo development, starch, soluble protein, soluble sugar, α-amylase, and antioxidant enzymes(SOD,POD, CAT) and MDA were closely related to seed dormancy release of Sarcandra glabra.更多还原