【作者】 张晔； 黄滢； 逄艳云； 张旭； 刘颖；

【Author】 Ye Zhang;Ying Huang;Yanyun Pang;Xu Zhang;Ying Liu;Department of Stomatology, Hospital of Stomatology, Tianjin Medical University;Institute of Stomatology, Tianjin Medical University;

【机构】 天津医科大学口腔医院；

【摘要】 目的:本研究旨在通过应用天然产物原花青素(PA),模拟糖胺聚糖(GAG)的优良生物功能。通过于纤维间形成稳定桥接结构,修复龋病条件下牙本质受损塌陷的胶原网。同时,利用PA的无机离子富集能力,协同性联合现有仿生再矿化策略,在稳定有机支架的基础上,强化对于脱矿牙本质的再矿化效果。此外,在本研究中同样评估了PA对于主要致龋菌的抑制作用,以期探索一种双功能牙本质龋防治策略。方法:通过应用红外傅立叶光谱验证PA于胶原纤维间形成的桥接结构,依据分子动态模拟,高分辨透射及扫描电子显微镜评估PA协同仿生矿化制剂对于胶原及脱矿牙本质的矿化修复效果。通过抗粘附及抗生物膜形成研究,评估PA对于多种致龋菌的抑制作用。最后,建立龋病动物模型,综合评判PA联同仿生再矿化制剂的抑菌及再矿化作用。结果:PA能够形成纤维间桥,从而支撑塌陷胶原网。此外,该GAG类似物可降低无机离子结合键能,在协同应用仿生再矿化制剂后,可明显促进脱矿牙本质仿生再矿化,使其机械性能接近天然牙本质。对于主要致龋菌,PA的抑菌效果可达95%以上。而在动物研究中,PA可有效抑制生物膜形成,并可联合仿生再矿化制剂于牙本质脱矿层重新形成再矿化屏障。结论:PA能够模拟GAG功能,修复受损塌陷的胶原支架,并可通过联合仿生再矿化制剂,提高牙本质的再矿化效率,对致龋菌活性同样展现了良好的抑制作用,为感染脱矿牙本质的仿生再矿化修复提供了一种极具前景的新策略。更多还原

【Abstract】 Objective:Glycosaminoglycans(GAG) form bridging cross-bands that maintain structural integrity of collagen in the organic matrix of dentine.The aim of this study was to evaluate the function of proanthocyanidins(PA) to simulate the bioactivities of GAG and observe the mineralization of collagen under the combined application of PA and CMC(carboxymethyl chitosan).Methods:PA combined with CMC were applied to collagen and demineralized dentine.The remineralization behavior was analyzed by means of fourier transform infrared spectra,scanning electron microscopy and transmission electron microscopy.Besides,the anti cariogenic effect of above complexes was also studied.Animal models were established for further detection.Results:Detailed analyses showed that PA reconstructed interfibrillar bridges of collapsed collagen network and reduced interfacial energy to enhance collagen hydrophilicity,subsequently promoting collagen mineralization.Besides,mechanical behaviors of demineralized dentine were significantly improved.PA exhibited satisfactory inhibitory effects on the activity of cariogenic bacteria.Moreover,ex vivo study indicated that remineralization and antibacterial effects were evident in the rat oral cavity.Conclusion:This strategy highlights the importance of maintaining structural integrity of the collagen network in dentine remineralization and offers great clinical potential for the effective treatment of dentine caries.更多还原