【作者】 袁晓君； 谢海峰； 张怀勤； 陈晨；

【机构】 南京医科大学附属口腔医院牙体牙髓科； 江苏省口腔疾病研究重点实验室； 江苏省口腔转化医学工程研究中心； 南京医科大学附属口腔医院修复科；

【摘要】 目的:评估10-甲基丙烯酰氧癸二氢磷酸酯(10-methacryloyloxydecyl dihydrogen phosphate,10-MDP)与甲基丙烯酰氧基丙基三甲氧基硅烷(methacryloxypropyltrimethoxysilane,γ-MPS)的相互作用及其对牙本质粘接耐久性的影响。方法:配置混合或不混合质量分数为0%、1%、5%、10%、15%的γ-MPS的质量分数为10%的10-MDP处理剂。牙齿酸蚀并处理后,使用粘接剂(Single Bond2,SB2)制备试件。水储24小时和12个月后,通过微拉伸强度(μTBS)测试及纳米渗漏评价粘接性能。原位酶谱评价基质金属蛋白酶(MMPs)活性。通过红外光谱(FTIR)、X射线衍射(XRD)与X射线光电子能谱(XPS)评估10-MDP与γ-MPS的相互作用。结果:12个月后,所有组的μTBS均显著降低(P<0.05)。混合质量分数为10%或15%的γ-MPS的10-MDP组的μTBS下降幅度大于混合质量分数为0%或1%的γ-MPS的(P<0.05),纳米渗漏更严重。相对于纯10-MDP组,混合质量分数为10%、15%的γ-MPS的荧光强度增加,10-MDP-钙盐特征峰的峰强度减小。结论:混合质量分数大于10%的γ-MPS会抑制10-MDP-钙盐形成,加剧MMPs激活,降低μTBS,增加长期的纳米渗漏,损害牙本质粘接耐久性。更多还原

【Abstract】 Objectives:The chemical interaction between 10-methacryloyloxydecyl dihydrogen phosphate(10-MDP) and methacryloxypropyltrimethoxysilane(gamma-MPS) and its effect on dentin bonding durability were investigated.Methods:Solutions containing 10 wt% 10-MDP and/or gamma-MPS at 0,1,5,10,or 15 wt% were prepared.Phosphoric-acid-etched dentine blocks were treated with primer followed by adhesive Single Bond 2 to build bonded specimens.After 24-hour or twelve-months water storage,the bonding properties were evaluated by micro-tensile strength(μTBS) test and nano-leakage.In situ zymography was used to investigate the activities of matrix metalloproteinases(MMPs).The interaction between 10-MDP and gamma-MPS was evaluated by Fourier-transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),and X-ray photoelectron spectroscopy(XPS).Results:Twelve-months water storage significantly lowered theμTBS for all the groups(p<0.05).The decrease for the groups containing 10 t% or15 wt% gamma-MPS was significantly greater than those for the groups containing 0 or lwt% gamma-MPS(p<0.05),showing more serious nano-leakage.Compared with the pure 10-MDP group,the groups containing 10 t% or 15 wt% gamma-MPS exhibited higher fluorescence intensities and lower peak intensities assigned to 10-MDP-calcium salts.Conclusion:The copresence of more than 10 wt% gamma-MPS inhibits 10-MDP-calcium salts formation and activates MMPs,leading to increased long-term nano-leakage and decreased bonding durability.更多还原