【作者】 佟美辰； 孙海博； 刘世杰； 牛卫东； 王丽娜；

【Author】 Tong Meichen;Sun Haibo;Liu Shijie;Niu Weidong;Wang Lina;Department of Stomatology, Dalian Medical University;

【机构】 大连医科大学口腔医学院；

【摘要】 目的:基于网络药理学分析苗药固齿方治疗牙周炎的作用机制,为临床治疗牙周炎提供参考。方法:通过检索文献和TCMSP数据库获取青盐,生石膏,续断,散血飞,对叶莲,骚羊古,艾纳香,卷柏,鸡屎藤的主要化学成分,设定OB≥30%,DL≥0.18为阈值,筛选中药活性成分,利用TCMSP靶点预测模型预测活性成分的潜在靶点;利用Genecards,Disgenet、TTD、DRUGBANK数据库检索牙周炎疾病相关靶点,将得到的活性成分作用靶点与牙周炎疾病作用靶点相结合,得到疾病-药物共同作用靶点,利用STRING数据库对获得的疾病-药物靶点构建PPI网络;利用Metascape平台,对疾病-药物靶点进行GO生物功能富集分析和KEGG代谢通道富集分析,而后采用Cytoscape3.7.1软件构建"苗药固齿方成分-牙周炎疾病靶点-通路"网络,最后通过Autodock Vina和PYMOL软件进行分子对接和可视化操作。结果:苗药固齿方治疗牙周炎的核心活性成分为槲皮素、木犀草素、山柰酚等;关键靶点有PTGS2、PTGS1、RELA、CASP3等;关键信号通路有AGE-RAGE信号通路、IL-17信号通路、PI3K-Akt信号通路等;其功能主要为调节炎症反应等。结论:本研究初步揭示了苗药固齿方通过多成分、多靶点、多途径治疗牙周炎的作用机制,为苗药固齿方的临床开发利用提供了依据。更多还原

【Abstract】 Objectives:To analyze the mechanism of treatment of periodontitis with Miao Medicine Guchi formula based on network pharmacology,and to provide reference for clinical treatment of periodontitis.Methods: By searching literatures and TCMSP Database to obtain the main compositin of the formula,OB≥30%,DL≥0.18 were set as thresholds to screen the active ingredients of Traditional Chinese medicine,and TCMSP target prediction model was used to predict the potential targets of the active ingredients.Genecards,Disgenet,TTD and DRUGBANK databases were used to search the targets related to periodontitis disease,and the disease-drug co-action targets were obtained by combining the obtained active ingredient targets with periodontitis disease targets.PPI network was constructed by using STRING database for disease-drug targets and potential protein functional modules in the network diagram were analyzed.Metascape platform was used to conduct GO biofunctional enrichment analysis and KEGG metabolic channel enrichment analysis for diseasedrug targets,and then Cytoscape3.7.1 software was used to construct a network of "components of Miao medicine guchi formula-periodontitis disease target-pathway".Finally,Autodock Vina and PYMOL software were used for molecular docking and visual operation.Results:The core active components of Miao medicine guchi formula in the treatment of periodontitis were quercetin,luteolin,kaempferol,etc.Key targets include PTGS2,PTGS1,RELA,CASP3,etc.Key signaling pathways include AGERAGE signaling pathway,IL-17 signaling pathway,PI3 K-Akt signaling pathway,etc.Its function is mainly to regulate inflammatory response and so on.Conclusion:This study preliminarily revealed the mechanism of action of Miao medicine guchi formula in the treatment of periodontitis by multiple components,multiple targets and multiple approaches,and provided a basis for the clinical development and utilization of Miao medicine guchi formula.更多还原