【作者】 狄飞倩； 石秀芹； 程文静； 李璐瑶； 张佳婵； 李萌； 王昌涛； 安全； 赵丹；

【Author】 Di Feiqian;Shi Xiuqin;Cheng Wenjing;Li Luyao;Zhang Jiachan;Li Meng;Wang Changtao;An Quan;Zhao Dan;College of Chemistry and Materials Engineering,Beijing Technology & Business University;Beijing Key Lab of Plant Resource Research and Development;Institute of Cosmetic Regulatory Science;Yunnan Baiyao Group Co.Ltd.;

【机构】 北京工商大学化学与材料工程学院； 北京市植物资源研究与开发重点实验室； 北京工商大学化妆品监管科学研究院； 云南白药集团股份有限公司；

【摘要】 氧化损伤会造成细胞衰老和凋亡,并最终导致器官或组织的老化和病变。不同的氧化损伤类型下细胞的自我调节和应答机制并非一致。本研究分别建立了UVA和H₂O₂诱导的人皮肤成纤维细胞氧化损伤模型,利用转录组测序（RNA-Seq）技术获得两种损伤模型下的差异表达基因（DEGs）,在完成功能注释和聚类分析的基础上,比较两种损伤下所参与信号通路的异同,并以文献报道的裂褶菌-谷物发酵活性物为验证对象,对关键基因进行mRNA水平验证。试验结果表明,DEGs主要集中在五种生物学过程:外部刺激反应、氧化应激、免疫、炎症和皮肤屏障调节;两种损伤模型中DEG富集所显示的生物学功能具有差异;PI3K-Akt信号通路在两种损伤模型中均发挥作用;模型及活性物作用下的关键基因表达水平验证试验证实了测序结果的准确性。本研究从分子水平比较分析了氧化损伤引起的基因和信号通路的变化,旨在建立氧化损伤评价标准,为今后活性成分的筛选和应用提供理论依据和技术支持。更多还原

【Abstract】 Oxidative damage is a focal factor that leads to skin aging by inducing cell damage and apoptosis.Existing studies have confirmed that both ultraviolet light and external environmental stimuli can cause oxidative damage to skin cells,but the molecular mechanism involved in cell damage and self-regulation has not been clearly elucidated.In this study,UVA and H₂ O₂ oxidative damage models were established,and the involvement of the PI3 K-Akt signaling pathway was evaluated by analyzing pathway-related differentially expressed genes（DEGs） in fibroblasts before and after injury using RNA highthroughput transcriptome sequencing（RNA-seq）.Finally,the fermentation of rice,oat and barley was conducted using a strain of Schizophyllum commune.Real-time quantitative PCR was used to verify the DEGs of the PI3 K-Akt signaling pathway and explore the repair activity of grain fermentation broth on oxidative damage in cells.The results showed that the DEGs were mainly enriched in five categories:external stimulus response,oxidative stress,immunity,inflammation,and skin barrier regulation.More importantly,the biological function shown by the enrichment of the DEGs in the two injury models were different,which confirmed the specificity of the mechanism of cellular oxidative damage caused by different factors.The cellular and molecular levels of the grain fermentation broth that could effectively repair oxidative damage generated by cells through the PI3 K-AKT pathway.This study comprehensively analyzed the change in the signaling trend and functional enrichment caused by oxidative damage at the cellular and molecular level,to evaluate the genes and pathways involved in the oxidative damage of human skin fibroblasts proved that the regulation of the PI3 K-Akt pathway in rice,oat,and barley.It is an effective way to relieve the oxidative stress of skin cells caused by ultraviolet light and external environmental stimulation,providing a basis for the future direction of research and related applications.更多还原