【作者】 刘静； 崔松鹤； 贺鹏霖； 丁姿羽； 郭赟； 马超； 朱婧； 谢志平； 刘源； 陈艳萍；

【Author】 Liu Jing;Cui Songhe;He Penglin;Ding Ziyu;Guo Yun;Ma Chao;Zhu Jing;Xie Zhiping;Liu Yuan;Chen Yanping;School of Agriculture & Biology,Shanghai Jiao Tong University;School of Life sciences and Biotechnology,Shanghai Jiao Tong University;

【机构】 上海交通大学农业与生物学院； 上海交通大学生命科学技术学院；

【摘要】 己醛是食品脂质氧化评估,果蔬成熟度检测和人类疾病早期诊断的重要挥发性化合物指标。因此,对己醛进行灵敏和选择性的监测是非常必要的。在此,我们从酵母细胞中异源表达嗅觉受体（MOR1-1）并利用含有受体纳米体作为识别元件特异性地结合己醛,并提出了一种基于ZIF-8@单壁碳纳米管（SWCNT）与纳米体-AuNPs （金纳米粒子）/普鲁士蓝（PB）相结合的固定化策略用于己醛的灵敏检测。ZIF-8@SWCNT作为双支撑材料为纳米体的负载提供了一个有效的高密度活性点区域,并增强了界面电荷转移。此外,利用AuNPs和PB良好的生物相容性和电化学活性,将纳米体-AuNPs/PB电沉积在ZIF-8@SWCNT功能化电极的表面,以进一步提高纳米体的固定化效率并放大电流信号。通过利用这一策略,嗅觉传感器表现出良好的特异性和灵敏度,对己醛的检测范围为10^-16～10^-9mol/L,检测限低至2.08×10^-17 mol/L。更重要的是,所设计的嗅觉生物传感器被成功地应用于评估不同葡萄发育阶段的成熟度。这种固定化策略在生物传感器的构建方面有很大的应用潜力。更多还原

【Abstract】 Hexanal is considered to be an important volatile compound indicator for the assessment of lipid oxidation in food,the determination of fruit and vegetable maturity,and the early diagnosis of human diseases.Therefore,the sensitive and selective monitoring of hexanal is highly desired.Herein,the nanosomes embedded with olfactory receptor（MOR1-1） derived from yeast were used as sensitive recognition elements to selectively bind hexanal.An efficient immobilization strategy integrated ZIF-8@Single-walled carbon nanotube（SWCNT） with Nanosomes-AuNPs（gold nanoparticles）/Prussian blue（PB）was proposed for sensitive detection of hexanal.ZIF-8@SWCNT as dual support materials provided an effective region of high-density active sites for nanosomes loading and enhanced interfacial charge transfer.Moreover,taking advantage of the excellent biocompatibility and electrochemical activity of AuNPs and PB,Nanosomes-AuNPs/PB was electrodeposited onto the surface of ZIF-8@SWCNT functionalized electrode to further enhance the immobilization efficiency of the nanosomes and amplify the current signal.By utilizing this strategy,the olfactory sensors exhibited excellent specificity and sensitivity,with a detection range of 10^-16-10^-9 mol/L and a low detection limit of 2.08 × 10^-17 mol/L for hexanal detection.More importantly,the designed olfactory biosensor was successfully applied to assess grape maturation during developmental stages.This immobilization strategy has promising potential applications in the fabrication of biosensors.更多还原