【作者】 吕孟敏； 白凤翎； 崔方超； 檀茜倩； 吕欣然； 励建荣； 郭晓华；

【Author】 Lü Mengmin;Bai Fengling;Cui Fangchao;Tan Xiqian;Lü Xinran;Li Jianrong;Guo Xiaohua;College of Food Science and Technology,Bohai University,National & Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University;Shandong Meijia Group Co.Ltd;

【机构】 渤海大学食品科学与工程学院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁省高等学校生鲜食品产业技术研究院； 大连工业大学海洋食品精深加工关键技术省部共建协同创新中心； 山东美佳集团有限公司；

【摘要】 苯乳酸是一种天然的具有广谱抑菌活性的小分子有机酸。本研究采用高效液相色谱(HPLC)法,以苯丙酮酸为底物从鱼肠道来源的乳酸菌中筛选高产苯乳酸的菌株,并借助单因素试验和响应面优化试验优化菌株的发酵条件,提高其合成苯乳酸的能力。结果表明,从50株乳酸菌中筛选到11株产苯乳酸的菌株,其中,菌株DY2合成苯乳酸能力较好,产量达到2.45 mg/mL,经形态学、生理生化和16S rDNA测序鉴定菌株DY2为融合魏斯氏菌(Weissella confuse)。通过单因素试验和响应面优化试验确定融合魏斯氏菌DY2最适发酵条件为:初始pH 6.0,初始接种量3%、发酵时间48 h,发酵温度34℃。在此优化条件下,融合魏斯氏菌DY2苯乳酸产量达到2.99 mg/mL,比优化前产量提高了1.22倍,以期为高产苯乳酸乳酸菌的工业化应用提供理论基础。更多还原

【Abstract】 Phenyllactic acid is a natural small molecule organic acid with broad-spectrum antibacterial activity.In this study,with phenylpyruvate as substrate,lactic acid bacteria(LAB) with high-producing phenyllactic acid was screened from fish intestines using high-performance liquid chromatography(HPLC).Fermentation conditions of LAB were optimized by single factor experiments and response surface optimization experiments for improving their ability of synthesize phenyllactic acid.The results showed that eleven strains LAB with producing phenyllactic acid were screened from fifty strains LAB.Among them,strain DY2 had good ability to synthesize phenyllactic acid with a yield of 2.45 mg/mL.The strain DY2 was identified as Weissella confuse by morphological,physiological and biochemical and 16 S rDNA sequencing.The optimal fermentation conditions of Weissella confuse DY2 were determined by single factor experiment and response surface optimization experiment as follows:initial pH 6.0,initial inoculum volume 3%,fermentation time 48 h,and fermentation temperature 34 ℃.Under this optimized condition,the yield of phenyllactic acid reached 2.99 mg/mL,which was 1.22 times higher than that before optimization,in order to provide a theoretical basis for the industrial application of high-yielding phenyllactic acid bacteria.更多还原