【作者】 王金洁； 徐如君；

【Author】 Wang Jinjie;Xu rujun;Department of Pathology,affiliated Hangzhou First People’s Hospital,Zhejiang University School of Medicine;

【机构】 浙江大学医学院附属杭州市第一人民医院病理科；

【摘要】 目的探讨弥漫大B细胞淋巴瘤中UCH-L1蛋白表达情况及其对临床病理特征和病人预后的影响。方法收集本院2014年至今存档石蜡包埋的DLBCL标本149例,按照Hans分型分为GCB和NonGCB两型,整理追踪病人的临床资料,6例反应性增生淋巴组织作为正常对照。免疫组织化学方法检测UCH-L1的表达并分析其与淋巴瘤分型和患者预后的关系,同时检测Bcl-2、Bcl-6和Ki-67等蛋白的表达情况。结果149例DLBCL患者中36例存在UCH-L1高表达,其中Non-GCB型16例(16/104,阳性率15%),GCB型20例(20/45,阳性率44%),两者差异具有统计学意义(P=0.000)。UCH-L1高表达与患者年龄(<60岁)有关,与性别无关(P值为0.018、0.552)。UCH-L1高表达的患者较低表达组具有更高的临床分期、LDH水平和IPI评分值(均P<0.05),而患者的白蛋白水平、有无B症状和骨髓累积在两组之间无明显差异。Bcl-2、Bcl-6和Ki-67的阳性表达率在UCH-L1高、低表达组间差异也无明显的统计学意义。结论UCH-L1高表达在GC源性DLBCL患者中更为普遍,其表达异常预示患者预后不良,它是一个良好的预后指标,对其表达进行抑制可能实现对DLBCL的有效基因治疗。更多还原

【Abstract】 Objective To investigate the expressions and prognostic impact of UCH-L1 in patients with diffuse large B-cell lymphoma(DLBCL) and its related factors.Methods One hundred and forty-nine paraffin specimens of DLBCL were collected from 2014 up to date,and classified into germinal center B-cell(GCB) type and NonGCB type according to Hans classification.Clinical information was collected and tracted.Six reactive hypertrophic lymphoid tissues were collected as a control.Immunohistochemical method was performed to detect the expression of UCH-L1 in these tissues.The correlation between UCH-L1 and the prognoses of these patients was analyzed.Also,the expression of Bcl-2,Bcl-6 and Ki-67 were detected.Results In all 149 DLBCL cases,UCH-L1 was overexpressed in 36 cases,including 16 cases in Non-GCB type(16/104,15%) and 20 cases in GCB type(20/45,44%),the difference was statistically significant(P=0.000).UCH-L1 expression was correlated with age of patients and not with gender.Higher clinical stage,LDH level and IPI score values were detected in UCH-L1 positive group compared with UCH-L1 negative group(P<0.05),while the albumin levels,B symptoms and bone marrow involvement were no obvious difference between two groups.The positive rates of Bcl-2,Bcl-6 and Ki-67 were also no difference between two groups.Comclusions Over-expression of UCH-L1 tended to appear in GCB type of DLBCL and was associated with poor outcome.It is a good prognostic indicator.Inhibition of UCH-L1 expression may achieve effective gene therapy for DLBCL..更多还原