【作者】 金婧彧； 吴紫茼； 锁然； 马倩云； 张彤； 冯佳齐； 王颉； 刘亚琼；

【Author】 Jin Jingyu;Wu Zitong;Suo Ran;Ma Qianyun;Zhang Tong;Feng Jiaqi;Wang Jie;Liu Yaqiong;College of Food Science and Technology,Hebei Agricultural University;

【机构】 河北农业大学食品科技学院；

【摘要】 本研究探究了不同超高压处理（200,400,600 MPa处理5或10 min）对海湾扇贝柱理化特性和蛋白质结构的影响。以新鲜的扇贝柱为对照组,考察了扇贝柱色泽、质地、微生物活性、显微结构及肌原纤维蛋白的总巯基与活性巯基、表面疏水性、Ca²⁺-ATP酶活性、粒径、热稳定性和电泳图谱等指标的变化。结果表明经超高压处理后,扇贝柱呈现较亮且不透明的外观,并且随着压力的增加a^*值和b^*值先降低后升高,其硬度和咀嚼性显著提高,弹性显著降低,微生物活性显著减少,肌纤维结构变得更加致密均匀。从扇贝柱中提取的肌原纤维蛋白的活性巯基和表面疏水性含量随压力增加而显著升高,而总巯基含量、Ca²⁺-ATP酶活性和平均粒径则显著降低。SDS-PAGE图谱显示出,与对照组相比肌动蛋白条带（42 ku）和肌球蛋白重链条带（220 ku）强度有轻微的降低。本研究表明,超高压处理能使扇贝柱蛋白质变性以及微生物活性的减少,更易于冷藏。更多还原

【Abstract】 The objective of this study was to investigate the effect of ultra-high pressure（UHP） treatment（200,400,600 MPa for 5 or 10 min） on the physicochemical characteristics and protein denaturation of the adductor muscle of the scallop（Argopecten irradians）（AMS）.With fresh AMS as the control,the changes of colour,texture,microbial activity,microstructure and the total and reactive sulfhydryl content,surface hydrophobicity,Ca²⁺-ATPase activity,mean particle size,thermal stability and electrophoresis pattern of myofibrillar protein were investigated.The results showed that after UHP treatments,AMS had a brighter and less translucent appearance,while a^* and b^* values decreased first,and then increased afterward,the hardness and chewiness was increased significantly,springiness,the microbial activity was significantly reduced and the muscle fiber structure becomes more dense and uniform.The reactive sulfhydryl and surface hydrophobicity content of myofibrillar proteins extracted from AMS were significantly increased following elevated pressure,while total sulfhydryl content,Ca²⁺-ATPase activity and mean particle size significantly decreased.SDS-PAGE showed a slight decrease in the intensity of the actin band（42 ku） and myosin heavy chain bands（220 ku） compared to the control group.This study showed that UHP treatment can denaturate the protein of AMS and reduce the microbial activity,which makes it easier to refrigerate.更多还原