【作者】 张健； 张祺悦； 李赫； 陈欣玮； 刘婉璐； 刘新旗；

【Author】 Zhang Jian;Zhang Qiyue;Li He;Chen Xinwei;Liu Wanlu;Liu Xinqi;Beijing Technology and Business University,National Suybean Processing Industry Technology Innovation Center;

【机构】 北京工商大学食品与健康学院国家大豆加工产业技术创新中心；

【摘要】 本研究旨在从富硒大豆肽中制备含硒大豆抗氧化肽,并研究其在体外氧化应激中的作用。富硒大豆肽通过凝胶过滤被分离成5个组分(F1～F5),并通过DPPH、ABTS和OH-自由基清除实验确定抗氧化活性最高的组分。进一步通过阴离子交换层析分离抗氧化活性最高的组分F4,得到F4-1～F4-5亚组分,通过DPPH、ABTS和OH-自由基清除试验评价其抗氧化活性。在F4-1亚组分中鉴定出序列为Ser-SeC-Ala-His-Lys(SSeCAHK)的含硒大豆抗氧化肽,并化学合成。测定SSeCAHK对过氧化氢诱导的HepG2细胞氧化应激的预防作用。SSeCAHK(0.13～0.50 mg/mL)预处理HepG2细胞2 h后,细胞内活性氧清除能力增强,同时防止了还原性谷胱甘肽含量下降和丙二醛含量增加。因此,SSeCAHK处理可改善过氧化氢诱导的HepG2细胞的氧化应激反应,表明SSeCAHK可作为膳食补充天然抗氧化功能材料,具有非常大的潜力。更多还原

【Abstract】 This study aimed to prepare selenium（Se）-containing soybean antioxidant peptides from Se-enriched soybean peptides（SSP） and determine their roles in oxidative stress in vitro.In this work,the SSP was separated into five fractions（F1 to F5）.Fraction F4,displaying the highest antioxidative activity,was further separated,and sub-fractions F4-1 to F4-5 were selected for antioxidative activity evaluation using DPPH,ABTS,and OH-radical scavenging assays.The Se-containing antioxidative peptides with sequence Ser-SeC-Ala-His-Lys（SSeCAHK） were identified in sub-fraction F4-1 and chemically synthesized.This Se-containing pentapeptide showed a preventive effect against hydrogen peroxide（H₂ O₂）-induced oxidative stress in HepG2 cells.Pretreating the cells for 2 h with SSeCAHK（0.13-0.50 mg/mL） induced strong intracellular,reactive oxygen species（ROS） scavenging activity while preventing a decrease in reduced glutathione（GSH） and an increase in malondialdehyde（MDA）.Therefore,SSeCAHK treatment improved H₂ O₂-induced oxidative stress in HepG2 cells,demonstrating the significant potential of SSeCAHK as a natural antioxidative functional material for dietary supplementation.更多还原