【作者】 陈燕； 朱玺燊； 杨雅婷； 浦仕献； 刘林； 周云莹； 李成云；

【Author】 Chen Yan;Zhu Xishen;Yang Yating;Pu Shixian;Liu Lin;Zhou Yunying;Li Chengyun;Yunnan Agricultural University, State Key Laboratory for conservation and Utilization of Bio-Resources in Yunnan;

【机构】 云南农业大学省部共建云南生物资源保护与利用国家重点实验室；

【摘要】 真菌感染的临床病例日益增多,每年新报道的致病真菌为8～10种。本研究采用扩增子测序技术检测了连续25个月采集的空气微生物样品,共注释到138种潜在的人类病原真菌,其中子囊菌门有64属115种,占总种数的83.3%,担子菌门4属16种,占比11.6%,接合菌门有4属7种,占比5.1%。与国内已有文献记录进行对比发现,其中仅48种我国已见报道,其余90种在我国尚未见报道,表明本研究所用方法更为灵敏,可检测更多病原真菌。通过GBIF查询这些真菌在全球的分布,发现大多数种类在许多国家和地区有分布记录,且分布记录的国家数与其在本研究中出现的频率和相对丰度均呈显著正相关。结果表明,利用此方法可获得大尺度上病原真菌的分布信息。通过多点连续监测,获得的数据可为空气传播的人类病原微生物的监测和人类健康风险评估及疾病的预防提供理论依据。更多还原

【Abstract】 The number of clinical cases of fungal infection is increasing, and 8 to 10 new pathogenic fungi are reported each year. In this study, ITS1 amplicon sequencing was introduced to detect 25 air microbial sam-ples which were consecutively collected from March of 2015 to June of 2017. As a result, 138 species of human pathogenic fungi were identified, including 115 species from 64 genera of Ascomycetes, accounting for 83. 3% of the total species, 16 species from 4 genera of Basidiomycetes, accounting for 11. 6%, and 7 species from 4 gen-era of Zygomycetes, accounting for 5. 1%. Compared with 181 species of fungi recorded in domestic medical my-cology books and literature, 48 of them have been reported in China, and the remaining 90 species have not been reported in China yet. The global distribution of these fungi was searched through GBIF, it was found that most species are widely distributed in the world, and the number of countries or areas with distribution records is significantly positive correlated with the frequency and relative abundance in the detection. However, whether these pathogenic fungi spreads widely through the air and causes disease is still unclear. Therefore, it is necessa-ry for medical fungi researchers and related workers to pay attention to potential human pathogenic fungi in air, especially those species have not been reported in China. Moreover, the study can provide methodological refer-ence for airborne pathogens monitor and offer theoretical basis for human health risk assessment and disease pre-vention. It will be more effective by continuously investigating in multisites.更多还原