【作者】 苏品璨； 何知航； 纪欣； 罗臻； 彭沫溱； 王志江； 姚富柱； 张新玉； 褚占光； 王珏；

【Author】 SU Pincan;HE Zhihang;JI Xin;LUO Zhen;PENG Mozhen;WANG Zhijiang;YAO Fuzhu;ZHANG Xinyu;CHU Zhanguang;WANG Jue;Yunnan Kunming Blood Center;Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College;Nujiang central blood station;

【机构】 云南昆明血液中心输血研究所； 中国医学科学院北京协和医学院输血研究所； 怒江州中心血站；

【摘要】 为了研究杀伤细胞免疫球蛋白样受体(KIR)与人类白细胞抗原(HIA)Ⅰ类在云南独龙族、白族、纳西族、彝族、壮族、傣族、苗族、傈僳族、哈尼族九个少数民族人群中的基因频率及配对分布。从云南无偿献血人群中选取1583名健康无关志愿者,采用聚合酶链式反应序列特异性引物扩增(PCR-SSP)进行KIR基因检测;采用聚合酶链式反应-直接测序分型法(PCR-SBT)进行HLA-A、B、C等位基因分型。KIR基因携带者频率和HLAA,-B和-C等位基因频率和群体中的配受体对数量以直接计数法计算。结果表明独龙族人群KIR2DL2和KIR2DS2的携带者频率与其他各组比较均存在显著差异(P<0.05),其他各个民族之间KIR携带频率未发现显著差异。独龙族人群HLA-Bw4频率与其他各组比较均存在显著差异(P<0.05),其他各个民族之间无显著差异。独龙族人群KIR2DL2+HLA-C1、KIR3DL1+HLA-Bw4、KIR2DS2+HLA-C1组合频率与其他各组比较均存在显著差异(P<0.05)。云南九个少数民族KIR基因和KIR/HLA配受体对分布情况存在不同程度的差异,独龙族相较其他云南八个民族具有独特的分布特征。更多还原

【Abstract】 The objectine of this paper is analyze the gene frequency and pairing distribution of killer cell immunoglobulin-like receptors(KIR) and human leukocyte antigen( HIA) Ⅰ in nine ethnic minorities in Yunnan, including Drung, Bai, Naxi, Yi, Zhuang, Dai, Miao, Lisu and Hani. A total of 1583 independent in-dividual cases were selected from the blood donors in Yunnan. KIR was genotyped by PCR-SSP. HLA-A,-B and-C loci were genotyped by PCR-SBT. KIR gene carrier frequency and HLA-A,-B and-C allele frequen-cy and the number of ligand receptor pairs in the population were calculated by direct counting method. The re-saltts show that there were significant differences in the carrier frequency of KIR2DL2 and KIR2DS2 in the Drung population compared with other groups(P<0. 05), while no significant difference was found in the carrier fre-quencies of KIR among other ethnic groups. There were significant differences in the frequency of HLA-Bw4 be-tween the Drung population and the other groups(P<0. 05), but there was no significant difference among other groups. There were significant differences in the combination frequency of KIR2DL2+HLA-C1, KIR3DL1+HLA-Bw4, KIR2DS2+HLA-C1 in the Drung population compared with other groups(P<0. 05). The distribution of KIR genes and KIR/HLA ligand receptor pairs in the nine ethnic minorities in Yunnan differs to varying de-grees. Compared with the other eight ethnic groups in Yunnan, the Drung ethnic group has unique distribution characteristics.更多还原