【作者】 麦麦提依明·托合提； 张诚； 高峰； 王继超； 黄啸元； 董军； 杨小朋； 吴永刚；

【Author】 MamateminTohti;Zhang Cheng;Gao Feng;Wang Jichao;Huang Xiaoyuan;Dong Jun;Yang Xiaopeng;Wu Yonggang;Department of Neurosurgery,The People’s Hospital of Xinjiang Uygur Autonomous Region;

【机构】 新疆维吾尔自治区人民医院神经外科；

【摘要】 目的探讨PEA3、EPHA2在胶质母细胞瘤侵袭性生长中的作用。方法构建U87细胞基因转染模型,将细胞系分为五组:空白组(control)、PEA3干扰组(si RNA-PEA3)、PEA3干扰空载组(NC-PEA3)、EPHA2干扰组(si RNA-EPHA2)、EPHA2干扰空载组(NC-EPHA2)。通过CCK8实验检测其增值率,细胞划痕实验检测其迁移能力,Transwell实验检测其侵袭能力。结果 CCK8实验结果显示与空白组相比PEA3干扰组和EPHA2干扰组的细胞增殖率明显下降(P<0.01、P<0.001),空白组与PEA3干扰空载组和EPHA2干扰空载组没有显著性差异。细胞划痕实验结果显示与空白组相比,PEA3干扰组和EPHA2干扰组的细胞迁移率下降,并与空白组有显著性差异(P<0.05),空白组与PEA3干扰空载组和EPHA2干扰空载组没有显著性差异。Transwell检测实验结果显示与空白组相比PEA3干扰组和EPHA2干扰组的侵袭细胞数明显下降(P<0.001),空白组与PEA3干扰空载组和EPHA2干扰空载组没有显著性差异。结论干扰PEA3基因和EPHA2基因能明显降低胶质母细胞瘤的增殖、迁移、侵袭能力。更多还原

【Abstract】 Objective To investigate the role of PEA3 and EPHA2 in invasive growth of glioblastoma.Methods The gene transfection model of U87 cells was constructed.The cell lines were divided into five groups:blank group(control),PEA3 interference group(siRNA-PEA3),PEA3 interference empty group(NC-PEA3),EPHA2 interference group(siRNA-EPHA2) and EPHA2 interference empty group(NC-EPHA2).The increment rate was detected by CCK8 test,the migration ability was detected by cell scratch test,and the invasion ability was detected by Transwell test.Results CCK8 experiment showed that compared with the blank group,the cell proliferation rate of PEA3 interference group and EPHA2 interference group decreased significantly(P<0.01,P<0.001).There was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group.The results of cell scratch test showed that compared with the blank group,the cell migration rate of PEA3 interference group and EPHA2 interference group decreased,and there was a significant difference with the blank group(P<0.05).There was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group.Transwell test results showed that compared with the blank group,the number of invasive cells in PEA3 interference group and EPHA2 interference group decreased significantly(P<0.001),and there was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group.Conclusion interference with PEA3 gene and EPHA2 gene can significantly reduce the proliferation,migration and invasion of glioblastoma.更多还原