【作者】 麦麦提依明·托合提； 张诚； 董军； 吴永刚； 杨小朋； Gao Feng； Abdukader； Sun Lianjie；

【Author】 MamateminTohti;Zhang Cheng;Gao Feng;Abdukader;Sun Lianjie;Dong Jun;Wu Yonggang;Yang Xiaopeng;Department of Neurosurgery,The People’s Hospital of Xinjiang Uygur Autonomous Region;

【机构】 新疆维吾尔自治区人民医院神经外科； Department of Neurosurgery,The People’s Hospital of Xinjiang Uygur Autonomous Region；

【摘要】 目的探讨mi R-124-3p在脑胶质母细胞瘤细胞系U87中的表达及其与肿瘤侵袭性的关系。方法利用脂质体载体转染技术对U87脑胶质母细胞瘤细胞株进行转染,建立高、中(对照组)、低表达3组不同mi R-124-3p表达量U87细胞系模型,通过实时荧光定量PCR检测三组细胞mi R-124-3p表达水平,用CCK-8实验研究其增殖能力。结果通过细胞转染技术转染后胶质母细胞瘤U87细胞系中miR-124-3p mimics及miR-124-3inhibitor稳定转染;实时定量PCR结果提示转染后空白对照组与mimics miR-124-3p表达量有显著统计学差异(P<0.01),miR-124-3p mimics与inhibitor表达量有显著统计学差异(P<0.01),空白对照组(正常组)与inhibitor miR-124-3p表达量有显著统计学差异(P<0.05)。CCK8增殖检测实验结果提示miR-124-3p mimics转染后U87细胞系增殖率降低,而miR-124-3p inhibitor转染后U87细胞系增殖率增高,差异有显著统计学意义(P<0.05)。结论 miR-124-3p在胶质母细胞瘤U87细胞系中抑制肿瘤细胞增殖活性。更多还原

【Abstract】 Objective to investigate the expression of mir-124-3p in human glioblastoma cell line U87 and its relationship with tumor invasiveness.Methods U87 glioma cell lines were transfected with Lipofectamine.The high,medium and low expression groups of mir-124-3p were established.The expression levels of mir-124-3p in the three groups were detected by real-time fluorescent quantitative PCR,and the proliferation ability was studied by CCK-8 experiment.Results after transfection,mir-124-3p mimics and mir-124-3inhibitor were stably transfected into human glioblastoma cell line U87.The results of real-time quantitative PCR showed that the expression of mir-124-3p in the blank control group was significantly different from that in the mimics group(P<0.01),and the expression of mir-124-3p mimics and inhibitor was significantly different(P<0.01) The expression of mir-124-3p was significantly different(P<0.05).The results of CCK8 proliferation test showed that the proliferation rate of U87 cell line was decreased after mir-124-3p mimics transfection,while the proliferation rate of U87 cell line was increased after mir-124-3p inhibitor transfection,the difference was statistically significant(P<0.05).Conclusion mir-124-3p can inhibit the proliferation of human glioblastoma cell line U87.更多还原