【作者】 苗艺缤； 林晓萍；

【机构】 中国医科大学附属盛京医院；

【摘要】 目的 :迁移到牙周组织中的巨噬细胞在牙周炎初期的炎症反应中有关键作用,本研究探索了青蒿素对牙周炎治病过程中的炎症抑制效果。方法 :通过将脂多糖溶液注入小鼠牙龈袋连续两天诱导牙周炎。青蒿素溶液与生理水混合后腹腔注射。通过免疫组织化学染色分析牙周膜中的巨噬细胞和破骨细胞浸润。通过westernblot测定牙龈组织中的IL-6和iκBα的蛋白水平。骨髓巨噬细胞在不同浓度的青蒿素环境下培养,同时使用脂多糖激活。RT-PCR检测促炎细胞因子IL-6和诱导型一氧化氮合酶(iNOS)的mRNA表达。iNOS蛋白水平和iκBα蛋白降解情况、信号转导子和转录激活子3(STAT3)磷酸化和核因子κB(NF-κB)通过免疫印迹和免疫荧光检测细胞核内转移情况。结果 :在培养24小时内,巨噬细胞IL-6和iNOS的m RNA水平在每个时间点都显著降低,差异有统计学意义(P<0.05)。诱导型一氧化氮合酶的蛋白表达,青蒿素组的iκBα降解水平和STAT3磷酸化水平降低。NF-κB的免疫荧光信号在青蒿素组的核区相对较弱。青蒿素组小鼠牙周膜巨噬细胞浸润和破骨细胞形成明显减少,差异有统计学意义(P<0.05)。结论 :青蒿素有助于抑制巨噬细胞的活化,这可能被证明对牙周炎的治疗有用。更多还原

【Abstract】 Objective: Periodontitis is a disease initiated by pro-inflammatory cytokines secreted by gingival macrophages in response to bacterial infection, leading to bone loss. In this study, the effects of artemether on the progression of periodontitis and macrophage function were evaluated. Methods: Periodontitis was induced by injecting lipopolysaccharide(LPS) solution into the gingival pocket of mice for two consecutive days. Artemether solution was mixed in physiological water and injected intraperitoneally. Macrophages and osteoclast infiltration in the periodontal ligament were analyzed by immunohistochemical staining. Protein levels of interleukin-6(IL-6) and inhibitor of NF-κB(iκBα) in gingival tissue were determined via western blot. Bone marrow macrophages were cultured under a series of artemether concentrations before activation with LPS. mRNA expression of proinflammatory cytokine IL-6 and inducible nitric oxide synthase(iNOS) was determined by RT-PCR. The protein expression of iNOS, iκBα degradation, signal transducer and activator of transcription 3(STAT3) phosphorylation, and nuclear factor kappa B(NF-κB) nuclear translocation in cells were detected via western blot and immunofluorescence. Results: Within 24 hours of incubation, macrophage mRNA levels of IL-6 and iNOS were significantly reduced at each time point. The protein expression of iNOS, degradation of iκBα, and STAT3 phosphorylation were reduced in the artemether group. The immunofluorescent signal of NF-κB in the nuclear region was weaker in the artemether group. The macrophages infiltration and osteoclast formation in the periodontal ligment were significantly reduced in artemether group mice. Conclusions: Artemether contributed to the inhibition of macrophage activation, which may prove useful in the treatment of periodontitis.更多还原