【作者】 鲁晓琪； 赵琦； 张丽娜； 李淑娟；

【Author】 Lu Xiao-qi;Zhao Qi;Zhang Li-na;Li Shu-juan;Department of Periodontology,Stomatological Hospital of Hebei Medical University;

【机构】 河北医科大学口腔医院牙周一科；

【摘要】 目的 :探究TNF-α和AGEs(advanced glycation end products, AGEs)能否诱导人牙龈间充质干细胞(hGMCs)发生氧化损伤,以便进一步探讨AGEs对hGMCs的性能的影响。方法 :酶消化法体外分离培养hGMCs,体外鉴定hGMCs表型分子鉴定,通过加相应的刺激在体外分别模拟健康、牙周炎、糖尿病、糖尿病伴牙周炎微环境,CCK-8检测其增殖,茜素红及油红O染液测定其成骨、成脂情况,流式细胞术检测ROS水平,丙二醛生成量,超氧化物歧化酶生成量检测其过氧化水平。结果 :与对照组相比,TNF-α-hGMCs组生成的ROS、MDA水平有所增加,T-SOD水平均下降,AGEshGMCs组和AGEs+TNF-α-hGMCs组的ROS生成量均显著增加,MDA水平均明显上升,T-SOD水平下降明显。结论 :AGEs和TNF-α都能抑制hGMCs的增殖活性和分化能力,诱导hGMCs发生氧化应激而生成内源性ROS,并导致hGMCs发生氧化损伤,但AGEs联合TNF-α作用对hGMCs造成的损伤比TNF-α更严重。更多还原

【Abstract】 Objective: To investigate whether TNF-α and AGEs can induce oxidative damage of human gingival mesenchymal stem cells(hGMCs.), so as to further explore the effect of AGEs on the performance of hGMCs. Methods: HGMCs cultivation in vitro enzyme digestion method, appraisal hGMCs phenotype in vitro molecular identification, by adding the corresponding stimulation in vitro simulated healthy,periodontitis, diabetes, diabetes with periodontitis microenvironment, CCK 8 testing its proliferation and alizarin red and oil red O determine its osteogenesis, into fat, flow cytometry detection level of ROS, mda production, The peroxide level was measured by superoxide dismutase production. Results: Compared with the control group, ROS and MDA levels were increased in TNF-α-hGMCs group, while T-SOD levels were decreased. ROS production was significantly increased in AGEs-hGMCs group and AGEs+TNF-α-hGMCs group, while MDA levels were increased significantly in both groups. The level of T-SOD decreased significantly. Conclusions: AGEs and TNF-α can inhibit the proliferation and differentiation ability of hGMCs, induce oxidative stress and endogenous ROS generation of hGMCs, and lead to oxidative damage of hGMCs, but AGEs combined with TNF-α can cause more serious damage to hGMCs than TNF-α.更多还原