【作者】 安丹； 蔡毓慈； 贺光磊； 薛翔； 赵艳； 谢林花； 吕政颐；

【Author】 AN Dan;CAI Yu-ci;HE Guang-lei;XUE Xiang;ZHAO Yan;School of Enviromental Science and Engineering, Shananxi University of Science &Technology;

【机构】 陕西科技大学环境科学与工程学院；

【摘要】 花生壳水解液转化为氢气的一个瓶颈问题是产氢效率低下。为解决这一问题,本文以花生壳水解液为碳源,采用"一步法"工艺将暗细菌与光合细菌置于同一个反应器中进行混合制氢实验,并对影响该混合菌群产氢特性的的主要条件,盐度、碳源浓度和产氢液初始pH进行优化研究。通过design expert 12.0设计15组实验,经Box-Behnken设计及响应面分析,获得最优制氢条件为:pH 8.42、碳源浓度6.505 g/L、盐度0.00 g/L,验证实验表明在该最优条件下暗细菌与光合细菌混合体系的产氢量为2345.15m L/L。本研究可为暗细菌与光合细菌混合制氢提供菌种资源和工艺条件参考。更多还原

【Abstract】 One of the bottlenecks in the conversion of peanut shell hydrolysate to hydrogen is the low hydrogen production efficiency. In order to solve this problem,peanut shell hydrolysate was used as carbon source, and dark bacteria and photosynthetic bacteria were put into the same reactor by "one-step" process for hydrogen production experiment. The main conditions affecting hydrogen production of the mixed bacteria, such as salinity, carbon source concentration and initial p H of hydrogen production solution, were optimized. 15 groups of experiments were designed by design expert 12.0. Through Box-Behnken design and response surface analysis, the optimal hydrogen production conditions were as follows: p H 8.42, carbon source concentration 6.505 g/L, salinity 0.00 g/L. The verification experiment showed that the hydrogen production yield of mixed hydrogen production system reached 2345.15 mL/L under the optimal conditions. This study can provide the reference of strain resources and process conditions for hydrogen production of mixed hydrogen production system.更多还原