【作者】 党国栋； 洪新宇； 蔡美琴；

【Author】 Dang Guo-dong;Hong Xin-yu;Cai Mei-qin;School of Public Health, Shanghai Jiao tong University;Shanghai Center for Disease Control and Prevention;

【机构】 上海交通大学医学院公共卫生学院； 上海市疾病预防控制中心；

【摘要】 目的研究烟酰胺单核苷酸（nicotinamide mononucleotide,NMN）对衰老小鼠能量代谢的影响及作用机制。方法将实验动物编号,根据衰老造模和NMN干预情况的不同,随机分为5组:正常对照组、早衰模型组、衰老模型组、干预1组、干预2组。检测内容包括小鼠体重、摄食量、脏器指数、能量代谢监测指标、糖耐量、胰岛素敏感性以及抗氧化水平。结果与对照组比较,模型组小鼠胸腺和肾脏指数降低,在夜晚的O₂消耗量、CO₂呼出量、活动能力和能量消耗显著下降,血糖水平显著升高,血清SOD、GSH-Px活性显著下降,MDA含量显著升高（均P<0.05）,验证D-gal衰老模型造模成功。与早衰模型组相比,干预1组在呼吸代谢、能量消耗、血糖水平等指标方面无明显差异（P>0.05）,活动能力和血清SOD、GSH-Px活性显著升高（P<0.05）,MDA含量显著降低（P<0.05）;与衰老模型组相比,干预2组夜晚的O₂消耗量、CO₂呼出量、活动能力和能量消耗显著增加（P<0.05）;血糖水平显著降低（P<0.05）,血清SOD活性显著升高（P<0.05）,血清及肝组织中的MDA含量显著降低（P<0.05）。结论给予衰老小鼠一定剂量的NMN连续进行6周及12周的干预,发现在能量代谢、糖耐量、胰岛素敏感性以及抗氧化水平等指标上有一定的改善,其改善能量代谢的作用机制很可能与提高细胞及组织的抗氧化能力有关。更多还原

【Abstract】 Objective To investigate the effects of Nicotinamide mononucleotide（NMN） on metabolism of aging mice and the related mechanism.Methods The experimental animals were randomly divided into 5 groups:normal control group, D-galactose model group and NMN intervention group;According to the aging modeling time of 6 weeks or 12 weeks, the mice of model group were divided into premature aging model group and aging model group. According to the intervention time of 6 weeks or 12 weeks. the mice of intervention group were divided into intervention group 1 and intervention group 2. Body weight, food intake, organ index, energy metabolism, glucose tolerance, insulin sensitivity and antioxidant level of mice were measured. Results Compared with the control group, the thymus index and renal index of model group were significantly decreased（P<0.05）.The O₂ consumption, CO₂ exhalation, energy consumption and activity ability of model group were significantly decreased at night（P<0.05）. The blood glucose level was significantly increased（P<0.05）. The activities of SOD and GSH-Px in serum were significantly decreased（P<0.05）, and the content of MDA in serum was significantly increased（P<0.05）. These indicate that the D-gal aging model has been successfully constructed. Compared with the premature aging model group, intervention group 1 had no significant difference in respiratory metabolism, energy consumption, blood glucose level and other indicators（P>0.05）. Activity ability was significantly improved（P <0.05）. The activities of SOD and GSH-Px in serum were significantly increased（P <0.05）. MDA content in serum was significantly decreased（P <0.05）. Compared with the aging model group, O₂ consumption,CO₂ exhalation, activity capacity and energy consumption in intervention group 2 were significantly increased at night（P<0.05）, but there was no significant difference between O₂ consumption and CO₂ exhalation in the daytime（P>0.05）. Blood glucose level was significantly decreased（P<0.05）.The activity of SOD in serum was significantly increased（P<0.05）. MDA content in serum and hepatic tissue was significantly decreased（P<0.05）.The activity of GSH-Px in serum and hepatic tissue had no significant difference（P>0.05）. Conclusion The energy metabolism, glucose tolerance,insulin sensitivity and antioxidant level of aging mice were improved by after 6 and 12 weeks of continuous intervention with a certain dose of NMN.The mechanism of action may be related to the improvement of antioxidant capacity of cells and tissue.更多还原