【作者】 王倩； 李政； 赵姗姗； 郄梦洁； 王明林； 郭军； 赵燕；

【Author】 WANG Qian;LI Zheng;ZHAO Shan-Shan;QIE Meng-Jie;WANG Ming-Lin;GUO Jun;ZHAO Yan;Institute of Agricultural Quality Standards & Testing Technology, Chinese Academy of Agricultural Sciences,Key Laboratory of Agricultural Product Quality and Safety, Ministry of Agriculture and Rural Areas;College of Food Science and Engineering, Inner Mongolia Agricultural University;College of Food Science and Engineering, Shandong Agricultural University;

【机构】 中国农业科学院农业质量标准与检测技术研究所； 内蒙古农业大学食品科学与工程学院； 山东农业大学食品科学与工程学院；

【摘要】 【目的】探究不同样品对肉羊产地溯源的准确度,通过比较不同部位羊肉中稳定同位素值,及对羊肉、全骨粉、脱脂骨粉与骨胶原的稳定同位素分析,为肉羊产地溯源鉴别方案提供有效参考指标。【方法】利用同位素比率质谱仪（IRMS）测定羊肉不同部位肌肉中δ¹³C、δ¹⁵N、δ²H和δ¹⁸O值,以及不同产地来源的羊肉、全骨粉、脱脂骨粉与骨胶原中δ⁽13C和δ⁽15N值,并对结果进行方差分析、线性判别分析与相关性分析,确定稳定同位素组成对不同样品来源的溯源能力。【结果】不同部位中δ¹³C、δ²H和δ¹⁸O值具有显著性差异,δ¹⁵N值在后腿、胸叉、排骨、腹腩和脖子五个部位间差异不显著,其中胸叉和腹腩中碳、氮、氢和氧稳定同位素值均无显著差异。不同产地的羊肉、全骨粉、脱脂骨粉与骨胶原中δ¹³C和δ¹⁵N值差异显著,羊肉对产地判别效果最佳,原始判别正确率为84.9%,交叉验证判别正确率为82.4%。全骨粉与骨胶原对产地判别正确率达65%以上。相关性分析结果表明羊肉、全骨粉、脱脂骨粉与骨胶原中δ¹³C、δ¹⁵N具有极显著相关性（P<0.01）,骨胶原与脱脂骨粉、全骨粉中碳同位素相关性最高,相关系数分别为0.903和0.866。【结论】稳定同位素可对不同来源的样品进行有效鉴别,并指示其地理来源,羊肉、全骨粉、脱脂骨粉及骨胶原中稳定同位素组成变化趋势基本一致,利用稳定同位素技术进行羊肉及羊骨的产地溯源是可行的。更多还原

【Abstract】 【Objective】The accuracy of different samples in tracing the origin of mutton was investigated. By comparing the stable isotope values of sheep meat in different parts and the stable isotope analysis of sheep meat,whole bone meal, defatted bone meal and bone collagen, so as to provide effective reference indicators for the traceability identification scheme of the origin of the sheep. 【Method】Isotope Ratio Mass Spectrometer（IRMS）was used to determine δ¹³ C, δ¹⁵ N, δ² H and δ¹⁸ O values in different parts of sheep meat, and δ¹³ C and δ¹⁵ N values in mutton, whole bone meal, defatted bone meal and collagen of the sheep bones from different origins. Analysis of variance（ANOVA）, linear discriminant analysis and correlation analysis were carried out on the results to determine the traceability of stable isotope composition to different sample origins. 【Result】The δ¹³ C, δ² H and δ¹⁸ O values in different parts have significant differences, but no significant difference in δ¹⁵ N values in hind legs,chest fork, ribs, abdomen and neck, and there was no significant difference in carbon, nitrogen, hydrogen and oxygen stable isotope values between the chest fork and abdomen. The δ¹³ C and δ¹⁵ N values of sheep meat, whole bone meal, defatted bone meal and bone collagen from different origins are significantly different. Mutton has the best effect on the origin discrimination, with the original discrimination accuracy rate was 84.9% and the cross-validation discrimination accuracy rate was 82.4%. The accuracy rate of distinguishing the origin by whole bone meal and collagen was over 65%. The correlation analysis results show that sheep meat, whole bone meal,defatted bone meal and bone collagen have extremely significant correlation（P<0.01）, and the carbon isotope in collagen had the highest correlation with defatted bone meal and whole bone meal, with correlation coefficients of 0.903 and 0.866, respectively. 【Conclusion】Stable isotope can effectively identify samples from different origins and indicate their geographical origins. The trend of stable isotope composition in mutton, whole bone meal,defatted bone meal and collagen is consistent. It is feasible to trace the origin of sheep meat and sheep bones using stable isotope technology.更多还原