【作者】 李一珂； 靳刚； 牛宇； 陈翊平； 谢孟峡；

【Author】 LI Yi-ke;JIN Gang;NIU Yu;CHEN Yi-ping;XIE Meng-xia;Analytical & Testing Center of Beijing Normal University;Institute of Mechanics,Chinese Academy of Sciences;University of Chinese Academy of Sciences;

【机构】 北京师范大学分析测试中心； 中国科学院力学研究所； 中国科学院大学；

【摘要】 蛋白质芯片技术因其高通量,微型化,可快速分析等特点,在生物检测中具有独特优势。光学蛋白质芯片同时结合了蛋白质芯片技术和高分辨的光学椭偏显微成像技术,在提高灵敏度的同时实现了检测结果的可视化。本工作利用光学蛋白质芯片技术,针对复杂体系中的临床标志物蛋白质,构建了夹心型检测体系,实现了系统的定量检测。实验结果与临床通用方法符合较好。该方法具有灵敏度高,操作简单,检测快速,样品用量少等优点。更多还原

【Abstract】 The protein chip technology has the merits of high-throughput,miniaturization,and fast analysis,playing an important role in biological detection.Optical protein chip combines the protein chip technology and the high resolution imaging ellipsometry,realizing the high sensitivity as well as the visualization.This work used the optical protein chip to achieve the quantitative detection of the clinical biomarker protein using the sandwich method.The results had good agreement with the commercial assay.Our lab-developed assay has the advantages of high sensitivity,simplicity,fast analysis,and small sample-consumption.更多还原