【作者】 丛丽丽； 曹方浩； 孙丹； 徐蔚青； 徐抒平；

【Author】 CONG Li-li;CAO Fang-hao;SUN Dan;XU Wei-qing;XU Shu-ping;State Key Laboratory of Supramolecular Structure and Materials,Institute of Theoretical Chemistry,Jilin University;Department of Chemistry,Zhuhai College of Jilin University;

【机构】 吉林大学超分子结构与材料国家重点实验室理论化学研究所； 吉林大学珠海学院化学系；

【摘要】 单细胞分析是目前一直备受关注的领域,通过对细胞群体中分离出来的个体细胞的研究来解释细胞的生理过程。单细胞的SERS检测也是近些年被关注的新领域,SERS检测单细胞的方法有几种,其中包括封片固定含有SERS底物的死细胞^[1]以及单个活细胞的SERS检测^[2]等。由于微流控技术具有高度集成、生成液滴的体积、速率可控等优势已经被应用于化学,生物学和纳米技术等的分析检测中^[3]。本研究结合微液滴技术与表面增强拉曼光谱,建立了原位检测单个细胞的方法,用于检测单个细胞表面的唾液酸（SA）。将Ag-MPBA纳米生物探针与细胞表面的唾液酸（SA）)结合,通过细胞结合探针个数以及光谱的SERS信号强度,识别正常细胞系与癌细胞系以及同种细胞系的单个活细胞表面的唾液酸（SA）含量的差异。更多还原

【Abstract】 Single-cell analysis is an area that has attracted much attention,and it is hoped that the physiological processes of cells will be explained through the study of individual cells isolated from cell populations.Single-cell SERS detection is also a new area of interest in recent years.There are several methods for detecting single cells using SERS,including the fixation of dead cells containing SERS substrate^[1] and SERS detection of single living cells^[2].The advantages of microfluidic technology,such as its high degree of integration,its ability to generate droplets,and the controllable rate,have been applied to the analysis of chemical,biological,and nanotechnologies^[3].This study combines micro-droplet technology with surface-enhanced Raman spectroscopy to establish a method for detecting single cells in situ for the detection of sialic acid（SA） on a single cell surface.Combining Ag-MPBA nanoparticles with sialic acid（SA） on the cell surface,the number of cell-binding probes and the SERS signal intensity of the spectrum identify the differences in cell surface sialic acid（SA） content in normal cell lines and cancer cell lines as well as the individual cells of the same cell line.更多还原