【作者】 鲍莹； 李洋； 国新华；

【Author】 BAO Ying;LI Yang;GUO Xin-hua;College of Chemistry,Jilin University;

【机构】 吉林大学化学学院；

【摘要】 蛋白质是生物体细胞和组织的主要组成部分,它通过形成一定的空间结构与其他分子作用而发挥功能。然而,受实验技术方法的限制我们对生物体中蛋白结构的认识非常有限。表面增强拉曼光谱（SERS）具有超高灵敏度,能够通过提供原子间相互作用的指纹图谱检测蛋白质的结构。我们实验室最近设计了一种新的SERS检测方法:用铝离子诱导银纳米粒子聚合产生热点,用于核酸结构的研究首次观察到了结构特征峰。在这个工作中,我们采用相同的纳米粒子对5种蛋白质（牛血清白蛋白、细胞色素C、肌红蛋白、过氧化氢酶、溶菌酶）进行SERS定量分析。信号增强效果比传统聚合体提高了约100倍,而且有较高的重现性。五种蛋白质的检出限可低至0.03ng·mL^-1。因此,我们相信该方法在蛋白分析中广泛应用前景。更多还原

【Abstract】 Protein is main component of the cells and tissues of the organisms.It functions by forming a certain spatial structure and interacting with other molecules.However,our understanding of the structure of proteins if organisms is limited with experimental techniques now.Surface enhanced Raman Spectroscopy（SERS） has highly sensitivity and can detect the internal structure of proteins by providing a fingerprint of interatomic interaction.We have designed a new SERS detection method to induce the aggregation of silver nanoparticles by aluminum ions and formation of the hot spot.The structural characteristic peaks were observed for the first time in the study of nucleic acid structure.In this work,we use this new method to quantitative analysis five proteins（bovine serum albumin,cytochrome C,myoglobin,catalase,lysozyme）,which can produce about 100 times more enhancement than conventional aggregators to obtain SERS spectrum with high reproducibility and sensitivity.The detection limit of five proteins can be as low as 0.03 ng·mL^-1.Therefore,we believe that this method will be used in protein analysis widely.更多还原