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适配体介导纳米银催化共振瑞利散射法测定水中痕量钾离子
Aptamer Mediated Nanosilver Catalytic Resonance Rayleigh Scattering Method for Determination of Trace Potassium Ion in Water
【Author】 LI Chong-ning;JIAN Xin;YAN Hui-hong;LIANG Ai-hui;JIANG Zhi-liang;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education,Guangxi Key Laboratory of Enviro nmental Pollution Control Theory and Technology,Guangxi Normal University;
【机构】 广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室;
【摘要】 在钾适配体(ssDNA)探针存在时,ssDNA吸附在纳米银表面,导致纳米银的催化作用弱,而当溶液中存在钾离子时,形成钾离子-ssDNA复合物,使ssDNA从纳米银表面脱附,此时纳米银得到释放,从而纳米银催化作用增强。随着钾离子浓度的增加,脱附的银纳米粒子越多,催化H2O2还原HAuCl4反应加快,随着钾离子加入量的增大,催化反应速率随之显著增强,生成的金纳米溶胶具有较高的共振瑞利散射(RRS)效应,导致体系在300 nm处的RRS强度线性增强。钾离子浓度在0~1.5μmol·L-1范围内,于300nm处的RRS强度增强值ΔI呈良好的线性关系。据此,可建立一种间接检测钾离子的RRS方法。
【Abstract】 Potassium adapter(ssDNA) adsorption on the surface of nanosilver,that lead to weak the catalysis.Wher the presence of potassium ions,the formation of K-ssDNA complexes,ssDNA from stripping on the surface of silver nanoparticles,nanosilver release right now,catalysis increase.With the increase of K+ concentration,the more silver nanoparticles stripping,H2 O2 catalyzed reduction of HAuCl4 reaction is accelerated,with the increase of potassium ion,catalytic reaction rate significantly enhanced the generated gold nanoparticle sol has higher RRS effect,lead to system of Rayleigh scattering intensity at 300 nm increase linearly.Potassium ion linear concentration is 0~1.5 μmol·L-1.Accordingly,a RRS method for potassium was established.
【Key words】 Potassium ions; Potassium-ssDNA complexes; Nano silver catalyst; Resonance Rayleigh scattering;
- 【会议录名称】 第二十届全国分子光谱学学术会议暨2018年光谱年会论文集
- 【会议名称】第二十届全国分子光谱学学术会议暨2018年光谱年会
- 【会议时间】2018-10-19
- 【会议地点】中国山东青岛
- 【分类号】TB383.1
- 【主办单位】中国光学学会、中国化学会、中国光学学会光谱专业委员会