【作者】 王仪涵； 姜晖； 王雪梅；

【Author】 Y Wang;H Jiang;X Wang;State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University;

【机构】 东南大学生物科学与医学工程学院生物电子学国家重点实验室；

【摘要】 与正常细胞相比,肿瘤细胞具有特殊的微环境,例如不同的氧化应激和较多的活性氧物种/还原性物种（ROS/RNS）,其过表达是肿瘤发生和恶化的重要原因之一^[1]。因此,监测细胞内ROS/RNS的变化可以提高对疾病临床表现的认识,有利于癌症的早期诊断,显著提高治疗效果^[2]。研究表明,荧光金纳米团簇可以在肿瘤细胞中原位生物合成,通过对生物体的生物响应识别及癌细胞的特定氧化应激微环境,并进一步用于靶向荧光生物成像和细胞内ROS检测^[3]。该方法操作简单,通过原位生物合成的金纳米簇具有良好的生物相容性,避免了传统化学合成相关纳米粒子的副作用。提出了一种基于微纳电极的细胞内电化学分析方法,用于单个肿瘤细胞内ROS的定量测定。结果表明,该检测平台具有较高的灵敏度,为定量跟踪单细胞水平的生物学功能和异质过程提供机会并用于精确的临床诊断和治疗。更多还原

【Abstract】 It is already known that compared with normal cells,cancer or tumor cells have a specific microenvironment and apparently possess a different oxidative stress & a relatively large amount of ROS/RNS,and their overexpression is one of the important reasons for tumor development and deterioration.Therefore,monitoring the changes of intracellular ROS/RNS can improve the awareness of the clinical manifestations of the disease,which will be benefit for the cancer early diagnosis and significantly improving treatment efficiency.Our recent research exploitation demonstrates that fluorescent gold nanoclusters could be readily biosynthesized in situ in cancer cells through bio-responsive recognition of the specific oxidation stress and micro environment of cancer cells,which could be further utilized for targeted fluorescent bio-imaging and intracellular ROS detection by using nanoelectrodes.This strategy has the advantages of simple operation and very good biocompatibility through the in situ biosynthesis of the gold,and avoids the side effects of relevant nanoparticles synthesized by traditional chemistry.A novel intracellular electrochemical analysis strategy based on micro/nano-electrodes was developed for the intracellular ROS quantitative determination in single cancer cells.The results show that the detection platform has high sensitivity.This may raise the possibility to open up new chances for quantitative tracking of biological functions and heterogeneous processes in single cell levels for precise clinical diagnostics and treatments.更多还原