【作者】 塔吉古丽·依马木买买提； 朱俊杰； 张剑荣；

【Author】 Tajiguli Yimamumaimaiti;Jun-Jie Zhu;Jian-Rong Zhang;State Key Laboratory of Analytical Chemistry for Life Science,Collaborative Innovation Center of Chemistry for Life Sciences,School of Chemistry and Chemical Engineering,Nanjing University;

【机构】 生命分析化学国家重点实验室南京大学化学化工学院；

【摘要】 酶生物燃料电池（EBFC）是理想的可植入型医疗器械的能量产生器,同时也是绿色的能源转换器^[1-4]。然而,未受保护的酶在人体血液中会被破坏,甚至失活。因此,开发出在人体血液中耐受血液中生物分子干扰的、稳定运行的生物酶催化剂对可植入式的酶生物燃料电池的发展十分重要。本工作创新性地创建了一种单壁碳纳米管（SWCNT）和级联酶-葡萄糖氧化酶（GOx）/辣根过氧化物酶（HRP）共嵌入亲水性MAF-7的生物催化剂（SWCNT-MAF-7-GOx/HRP）。该生物催化剂在高温和分子抑制剂的作用下,仍保持高稳定的电催化活性。此外,我们发现GOx/HRP在亲水性SWCNT-MAF-7中的电催化活性远远超过GOx/HRP在疏水性SWCNT-ZIF-8中的电催化活性。在人类全血中,与无保护的EBFC相比,SWCNT-MAF-7-GOx/HRP催化的EBFC的功率密度增加了8倍(119μW cm^-2 vs 14μW cm^-2),稳定性提高了13倍。本研究成功地实现了金属-有机框架封装技术在生物燃料电池领域的应用,为可植入式生物发电装置的研制开辟了新途径。更多还原

【Abstract】 The EBFC has been considered as a promising implantable energy generator because it can extract energy from a living body without any harm to the host.However,an unprotected enzyme will be destabilized and even eventually be deactivated in human blood.Thus,the performance of implantable EBFC has received barely any improvement.It is therefore a breakthrough in realizing a superior efficient EBFC that can work stably in human blood which relies in protecting the enzyme to defend it from the attack of biological molecules in human blood.Herein,we innovatively created a single-walled carbon nanotube（SWCNT） and cascaded enzyme-glucose oxidase（GOx）/horseradish peroxidase（HRP） co-embedded hydrophilic MAF-7 biocatalyst（SWCNT-MAF-7-GOx/HRP）.The SWCNT-MAF-7-GOx/HRP is highly stable in electrocatalytic activity even when it is exposed to high temperature and some molecular inhibitors.In addition,we were pleasantly surprised to find that the electrocatalytic activity of GOx/HRP in hydrophilic SWCNT-MAF-7 far surpasses that of the GOx/HRP in hydrophobic SWCNT-ZIF-8.In human whole blood,the SWCNT-MAF-7-GOx/HRP catalytic EBFC exhibits an eightfold increase in power density(119 μW cm^-2 vs 14 μW cm^-2) and 13-fold increase in stability in comparison with the EBFC based on an unprotected enzyme.In this study,the application of metal-organic framework-based encapsulation techniques in the field of biofuel cells is successfully realized,breaking a new path for creating implantable bioelectrical-generating devices.更多还原