【作者】 江宇静； 姜立萍； 张剑荣； 朱俊杰；

【Author】 Yujing Jiang;Li-Ping Jiang;Jian-Rong Zhang;Jun-Jie Zhu;State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University;

【机构】 南京大学化学化工学院生命分析化学国家重点实验室；

【摘要】 单个产电菌的表面修饰代表了微生物燃料电池（microbial fuel cells,MFCs）的巨大发展,该策略确保每个细菌细胞都可以与阳极材料保持紧密接触,从而提高性能[1-3]。本文中,构建了Au和Fe₃O₄共修饰的S.oneidensis MR-1,用于MFCs的生物阳极。共修饰的细菌细胞是通过Au纳米颗粒的生物矿化和Fe₃O₄纳米颗粒的静电吸引而复合的。高导电的金纳米颗粒加速了生物膜内部以及生物膜/电极界面之间的电子转移[4]。同时,生物亲和性的Fe₃O₄可以长时间保留细菌的代谢活性[5]。更重要的是,来自Fe₃O₄纳米颗粒的磁性可以使共修饰的产电菌快速富集到磁性基底电极上,从而大大提高了细菌的负载量并减少了细菌附着的时间。三功能修饰的产电菌所构建的磁辅助生物阳极在启动速度,稳定性和输出性能方面远远优于天然细菌。这项工作不仅加深了细胞表面修饰技术在MFCs中的应用,而且使细菌细胞进入了多重修饰阶段。更多还原

【Abstract】 Surface modification of exoelectrogenic bacteria has represented an impressive development in microbial fuel cells（MFCs）,because every bacterial cell can keep an intimate contact with anodic material for upgrading the performance[1-3].Here,the Au and Fe₃O₄ co-modified Shewanella oneidensis MR-1 has been developed for the bioanode of MFCs.The co-modified bacterial cells are synthesized by the biomineralization of Au nanoparticles（NPs） and the electrostatic attraction of Fe₃O₄ NPs onto the cell surfaces.The high-conductive Au NPs accelerate the electron transfer inside biofilm and between the biofilm/electrode interfaces[4].Meanwhile,the bioaffinity Fe₃O₄ NPs can retain the bacteria metabolic activity for a long time[5].More importantly,the magnetic activity from Fe₃O₄ NPs allows the fast capture of co-modified bacteria onto the magnetic substrate electrode,largely improving the bacterial loading amount and decreasing the time for bacterial colonization.As a result of this trifunctional modification,the magnet-assisted bioanode fabricated with co-modified bacteria is far superior to that with native bacteria in start-up,stability and output performance.This work not only deepens the application of cell-surface modification technology in MFCs but also bring bacterial cells into the stage of multi-step modification.更多还原