【作者】 吴叶宇； 冯德芬； 韦富存； 谭学才；

【Author】 Defen Feng;Fucun Wei;Yeyu Wu;Xuecai Tan;School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry;

【机构】 广西民族大学化学化工学院广西高校食品安全与药物分析化学重点实验室广西林产化学与工程重点实验室；

【摘要】 本工作发展了一种基于MIL-53（Al）@CdS QDs和SiO₂@AuNPs的电化学发光生物传感器用于敌百虫的检测。金属-有机骨架（MOFs）被用作负载基质,为电极的修饰材料和目标物的负载提供较大的比表面积。同时,SiO₂@AuNPs负载了大量的AuNPs颗粒,可以有效的增强AuNPs与CdS量子点之间的ECL共振能量转移,因此ECL响应被抑制并达到信号放大的效果。在敌百虫的浓度在10^-11-10^-4 M的范围内,ECL的响应信号与浓度的对数呈线性关系,检出限位5.1×10^-12 M （S/N=3）。将此电化学传感器用于实际样品生菜、西兰花、黄瓜和韭菜中敌百虫的检测,所得加标回收率分别为97%-105%,102%-104%,100%-104%,和98%-104%。综上,此新型ECL传感器具有良好的检测效果,有望用于食品中敌百虫的检测。更多还原

【Abstract】 An ultrasensitive electrochemiluminescence （ECL） biosensor was developed based on MIL-53（Al）@CdS QDs and SiO₂@AuNPs for trichlorfon detection.Metal-organic frameworks （MOFs） were used as a loading platform that provided a large surface area to load targets and modified materials onto the electrode.At the same time,SiO₂@AuNPs loaded plenty of AuNPs which effectively increased ECL resonance energy transfer between CdS QDs,so the ECL signal was inhibited and made signal amplified.In the range of10^-11-10^-4 M,the ECL response presented linear relationship to the concentration （logarithm） of trichlorfon,and the detection limit is 5.1×10^-12 M （S/N=3）.When the biosensor was applied to the detect trichlorfon in lettuce,broccoli,cucumber,and chives,the recoveries obtained from spiked samples were 97%-105%,102%-104%,100%-104%,and 98%-104%,respectively.Thus,this novel ECL biosensor presented a potential application for the analysis of trichlorfon in food samples.更多还原