【作者】 Meiqi Zhang； Jiayuan Zhang； Yuxin Song； 王晶；

【Author】 Meiqi Zhang;Jiayuan Zhang;Yuxin Song;Jing Wang;State Key Laboratory of Natural and Biomimetic Drugs,Department of Chemical Biology,School of Pharmaceutical Sciences,Peking University;

【机构】 State Key Laboratory of Natural and Biomimetic Drugs,Department of Chemical Biology,School of Pharmaceutical Sciences,Peking University； 北京大学；

【摘要】 The interconversion of guanosine triphosphate（GTP） and guanosine diphosphate（GDP） is integral to a wide variety of biological cellular activities^[1].However,analytical methods which directly detect the ratio of intracellular GTP and GDP concentrations have not been available.Herein,we report GNEPS,a genetically encoded fluorescent sensor that enables real-time monitoring of the GTP:GDP ratio,which is a fusion protein comprising a eukaryotic G-protein and a circularly permuted yellow fluorescent protein.GNEPS has distinct fluorescence spectra between its GTP-bound and GDP-bound states.Its apparent fluorescence signal therefore depends upon the competitive binding of GTP and GDP.Live cell imaging experiments demonstrated that GNEPS can be used to monitor spatiotemporal changes in the intracellular GTP:GDP ratio in various cell types and organelles in response to metabolic perturbations^[2].We anticipate that GNEPS will become a valuable tool for understanding the metabolic and regulatory contributions of guanosine nucleotides.更多还原

【Abstract】 The interconversion of guanosine triphosphate（GTP） and guanosine diphosphate（GDP） is integral to a wide variety of biological cellular activities^[1].However,analytical methods which directly detect the ratio of intracellular GTP and GDP concentrations have not been available.Herein,we report GNEPS,a genetically encoded fluorescent sensor that enables real-time monitoring of the GTP:GDP ratio,which is a fusion protein comprising a eukaryotic G-protein and a circularly permuted yellow fluorescent protein.GNEPS has distinct fluorescence spectra between its GTP-bound and GDP-bound states.Its apparent fluorescence signal therefore depends upon the competitive binding of GTP and GDP.Live cell imaging experiments demonstrated that GNEPS can be used to monitor spatiotemporal changes in the intracellular GTP:GDP ratio in various cell types and organelles in response to metabolic perturbations^[2].We anticipate that GNEPS will become a valuable tool for understanding the metabolic and regulatory contributions of guanosine nucleotides.更多还原