【作者】 夏佳睿； 贾谊； 李峻柏；

【Author】 Jiarui Xia;Yi Jia;Junbai Li;Beijing National Laboratory for Molecular Sciences (BNLMS),Institute of Chemistry,CAS Key Lab of Colloid,Interface,and Chemical Thermodynamics,Chinese Academy of Sciences;

【机构】 胶体界面与化学热力学实验室中国科学院化学研究所；

【摘要】 分子仿生是以生物分子、生物杂化分子以及人工分子为研究对象,在分子水平上实现对生物体系微纳尺寸的功能性结构与材料的仿生。本文报告以驱动蛋白-微管体系为研究对象,研究分子仿生的自组装以及分子马达体系,通过构筑或模拟具有物理化学或是生物功能的组装体并进行人工调控,使得驱动蛋白-微管体系的运动限域控制,都可以有望最终实现对生物体或生命过程的模拟。本文报告设计了一种通过构建二维(2D)基底亲疏水性来控制驱动蛋白带动微管定向运动的系统,首次在胺基修饰玻璃表面引入二碳酸叔丁基酯((Boc)2)来构建2D轨道,进行(Boc)2保护后基底的亲疏水性可以被逆转,从而使表面从亲水性转变为疏水性,通过对基底选择性处理,可使上述基底在特定区域实现亲疏水性分区。通过在基底上标记纳米粒子或荧光染料可以有效地观察到亲水性区域,并且基底中的亲水部分将允许驱动蛋白选择性地固定在该表面,从而控制微管的定向运动。这种2D轨道的设计和选择性修饰在生物分子马达体系的纳米器件领域具有潜在的应用价值。更多还原

【Abstract】 As for the reasearch of organism and life, it is important to achieve the designand synthesis of biomimetic micro-nano materials with specific functionality via self-assembly at the molecular level. The formed biomimetic molecular assemblies can not only simulate organisms to achieve multi-functional integration and correlation, but also lead to the preparation of functional materials, molecular machines and biomedical materials or devices with biological compatibility and functions. Therefore, the development of biomimetic micro-nano materials can pave the way for unlimited innovation and development of modern material science. Aiming to that, molecular motor systems were systemetically studied by constructing or simulating the self-assembly of kinisin-microtubule in this thesis. Based on a patterning method, a novel system for guiding directional and restricting movements of kinesin-driven microtubule on a two-dimensional(2 D) substrate was established. The conventional t-butoxycarbonyl(Boc)2 O protection was firstly introduced to treat the amine modified glass surface to construct 2 D track. The polarity of glass surface can be efficiently reversed after Boc protection, resulting in the surface transferring from hydrophilicity to hydrophobicity. Based on a subsequent selective treatment of Boc de-protection, the substrate surface can be well distributed into hydrophilic and hydrophobic partitions. Such method is of great flexible to construct hydrophilic pattern with designed shapes. Moreover, the hydrophilic areas can be visualized via the labeling of nanoparticles or fluorescent dye. Such hydrophilic pattern at the hydrophobic background rendered kinesin to be well selectively immobilized on this surface, thus resulting in the effective control of restricted or directional microtubule movement. With this design, the construction of 2 D tracks may have a potential application in field of biomolecular motors based nanodevice.更多还原