【作者】 刘杰； 鹿洁； 于丽；

【Author】 Jie Liu;Jie Lu;Li Yu;Key Laboratory of Colloid and Interface Chemistry,Shandong University,Ministry of Education;

【机构】 山东大学胶体与界面化学教育部重点实验室；

【摘要】 采用稀土铕包覆的多金属氧化簇Na₉EuW₁₀O₃₆·32H₂O（Eu-POM）和壳聚糖（CS）制备了Eu-POM/CS复合薄膜。通过肉眼观察和紫外-可见分光光度计表征发现该壳聚糖复合薄膜具有较高的透明度。傅里叶变换红外（FTIR）光谱图表明CS和Eu-POM的结构在所制备的复合薄膜中都得到了良好的保持。值得一提的是,Eu-POM/CS复合薄膜不论是在荧光寿命还是在荧光强度方面相比于Eu-POM都有了较大的提升。如图1所示,复合薄膜的荧光强度随着双氧水浓度的增大而逐渐减弱,发现浓度仅为0.11μM的双氧水对复合薄膜的荧光猝灭效率高达70%。复合薄膜的荧光强度与双氧水的浓度（浓度范围为1.1～66μM）之间有着良好的线性关系,检测限为1.1μM。基于此,我们将该复合薄膜应用于检测双氧水,发现该方法展现了良好的稳定性和较高的灵敏度。血清中的多种成分对复合薄膜检测双氧水没有明显干扰,表明该种方法具有良好的选择性。可见,Eu-POM/CS复合薄膜可以便捷、灵敏、无标记地检测生物样品中的双氧水,对相关疾病的诊断具有一定的指导意义。更多还原

【Abstract】 Eu-POM/CS composite films were fabricated from europium-containing polyoxometalates（Eu-POM） and chitosan （CS）.Both observation with the naked eye and characterization with UV-vis spectrometer addresses the high transparency of the as-prepared films.In contrast to pure Eu-POM,the fluorescence intensity and fluorescence lifetime of the Eu-POM/CS films are drastically enhanced.As shown in Fig.1,with the increasing concentration of hydrogen peroxide （H₂O₂）,fluorescence intensity of the Eu-POM/CS films decreases gradually.Fluorescence quenching efficiency is up to 70%even the concentration of H₂O₂ is only 0.11μM.There is a good linear correlation between the fluorescence intensity of Eu-POM/CS and H₂O₂ concentration within a wide range of 1.1-66μM and the detection limit is estimated to be 0.11μM.Based on this,the composite films display a high sensitivity and stability for H₂O₂ detection.The composite film also has an excellent selectivity for H₂O₂ detection even in the presence of extremely complex components in serum.It can be seen that Eu-POM/CS composite films can detect H₂O₂ in biological samples and have certain guiding significance for the diagnosis of related diseases.更多还原